The Correlation Research Between SPLA2 Gene Single Nucleotide Polymorphism And Coronary Heart Disease

Objective: By exploring the level of secretory phospholipase A2 type and its 1640C>T gene single nucleotide polymorphisms characteristics in patients with coronary heart disease,we try to investigate the relatio nship between the genetic susceptibility of PLA2G5 gene polymorphism and CHD,and s PLA2.Methods: A case-control study method was adopted. 282 patients with CHD, confirmed by coronary angiography,and 110 patients without CHD are divided into CHD group and control group. The basic data collected contain gender, age, CRP, blood lipids and so on. And we extract the peripheral venous blood,which were measured the serum concentration of s PLA2 with enzyme-linked immunosorbent assay,and DNA were extracted with the polymerase chain reaction-restriction fragment length pollymorphism(PCR-RFLP).Then we made comparison between different groups of 1640C>T alleles distribution characteristics and analyzed its single nucleotide polymorphisms and susceptibility to coronary heart disease and the relationship between the clinical indicators.Result: 1.The serum secretory phospholipase A2 of CHD patients are obviously higher than that of control group,and the difference have statistical significance.2.The genotype distribution of PLA2G5 1640C>T gene polymorphism are consistent with Hardy-Weinberg equilibrium law.3.three types of CHD group are GG,GA and AA,which distribution frequency are 0.600,0.320 and 0.080.The distribution of frequency in the control group,in turn,are 0.45,0.42 and 0.12.Genotype distribution between the two group is statistically different(χ2=6.591,P=0.037).4.Coronary heart disease group G,A frequency distribution of alleles are 0.521,0.479,in the control group are 0.759,0.241. The allele frequency distribution difference between the two groups is statistically significant(χ2=6.702,P=0.031).5.Thepatients in group of GG、GA、AA are obviously different in diabetes history,LDL,HDL and Gensini score.6.Multiariable logistic regression analysis showed that people with A alleles got risk of CHD are 1.557 times than those withou the allele,and the allele of A is independent risk factor for coronary heart disease(O R=1.557,P=0.048,95%CI=1.479~1.639).Conclusion: 1.Serum phospholipase A2 level are significantly increases in patients with coronary heart disease.2.In patients with coronary heart disease,sec retory phospholipase A2 1640C>T gene introns exist three different genotypes and the genotype distribution are obvious different with the distribution with the contro l group.3.The single nucleotide polymorphisms of secretory phospholipase A2 type V gene introns 1640C>T relate to the onset of CHD and allele A may be a genetic susceptibility genes of coronary heart disease pathogenesis.