Effect Of Different Amount Of Thermal Stimulation On Bronchioles Morphology Of Asthma In New Zealand Rabbits By Dog Days Moxibustion

Objective:To provide experimental evidence for improving clinical effect, we observe the effect on chest temperature and bronchioles morphology in New Zealand rabbit by dog days moxibustion with different amounts of moxa cones, and further explore the possible mechanism that affect the efficacy of asthma.Methods:Select 30 male New Zealand rabbits, body mass:(2.5±0.3)kg. They were randomly divided into five groups:blank control group、model control group、 dexamethasone control group、 three moxa cones control group and nine moxa cones control group. Each group has 6 New Zealand rabbits. Every group was made into asthmatic airway remodeling model except the blank control group. We gave different intervention to dexamethasone control group、three moxa cones control group and nine moxa cones control group during manufacture asthmatic airway remodeling model. The dexamethasone control group were administered intragastrically with dexamethasone, three moxa cones control group and nine moxa cones control group were intervented in moxibustion on ginger and external application of Chinese medicine in dog days and we used infrared thermography to monitor the chest temperature of New Zealand rabbits of three moxa cones control group and nine moxa cones control group. We did all of this every other day,15 times in all. Then, all of the New Zealand rabbits were killed and we took out their right lung for HE staining. At last we compared the difference of bronchioles morphology of right lung in each group and compared the difference of temperature of New Zealand rabbits of three moxa cones control group and nine moxa cones control group.Results:(1) From the morphology, the thickness of bronchial smooth muscle and bronchial wall have significant difference between model control group and blank control group (P<0.05). (2) The thickness of bronchial smooth muscle and bronchial wall have no significant difference between moxibustion three moxa cones control group and modle control group (P>0.05); the thickness of bronchial smooth muscle and bronchial wall have significant difference in nine moxa cones control group, three moxa cones control group and modle control group (P<0.05); the thickness of bronchial smooth’muscle and bronchial wall have significant difference in dexamethasone control group, three moxa cones control group and modle control group (P<0.05), but have no significant between dexamethasone control group and nine moxa cones control group (P>0.05). (3) After moxibustion, the chest temperature of New Zealand rabbits of three moxa cones control group and nine moxa cones control group have significant increase (P<0.05) but nine moxa cones control group is higher than three moxa cones control group (P<0.05) and the temperature rise times of nine moxa cones control group is more than three moxa cones control group,There is significant difference between nine moxa cones control group and three moxa cones control group before and after moxibustion (P<0.05).Conclusion:(1) The thickness of bronchial smooth muscle and bronchial wall of model control group is thicker than blank control group. It suggests that the experimental method of making model is successful. (2) The bronchioles morphology of nine moxa cones control group is better than model control group and three moxa cones control group and well-matched with dexamethasone control group. This indicates that nine moxa cones control group can significantly improve the airway remodeling phenomenon and have better efficacy in dog days moxibustion. (3)Our experimental result suggests that the extent of temperature elevate after moxibustion is associated with the effect of dog days moxitustion and the efficacy of high temperature group is better than low temperature group. (4) In summary, increasing the amount of dog days moxibustion can raise the chest temperature of New Zealand rabbits and reach a certain threshold, and then induce "qi extending affected parts" phenomenon. This phenomenon can help bronchioles morphology of asthma New Zealand rabbits to become better. As a result, It can improve airway remodeling phenomenon significantly and achieve therapeutic effect.