| Objective:To observe the temperature of meridian points surface in cold stagnation syndrome of dysmenorrhea rats and the LTB4 amount of uterus and the effect of moxibustion, to compare the characteristics between the local and distal acupuncture points.Methods:Forty eight female SD rats were evenly randomized into control, model, Sanyinjiao(SP6), Guanyuan(RN4) groups. Model was established in the latter three groups by subcutaneous injection of estradiol benzoate at 0.5 mg for 10 successive days and lh after the last administration, intraperitoneal injection of oxytocin at 2U,followed by exposure of the rats to -25 ℃ in a freezer at 4h per day for 5 days.Meanwhile, the control rats received saline only and were not exposed to the low temperature. Infrared thermal imaging was used to measure the skin temperature at the uterine body surface before and Omin,5min, lOmin, 20min,30min after moxibustion.Results:1.Comparation of uterus time point temperature:The temperature change of the different points among the four groups was statistically significant(P<0.05).The interaction of the group and the monitoring time were statistically significant(P<0.05).Infrared temperature difference among four groups had statistical significance(P<0.05)Among them, there was no significant difference on each point in normal group. Each time during 20-30 min after moxibustion, the temperature of model group all decreased significantly(P<0.05) and each point from 30～60 min decreased significantly than that immediate after moxibustion (P<0.05).Each time during 5～30 min, the temperature of Sanyinjiao(SP6) group decreased significantly than that before moxibustion (P<0.05) and from 10～60 min decreased significantly than that immediate after moxibustion(P<0.05). On immediate after moxibustion, the temperature of Guanyuan(RN4) group elevated significantly(P>0.05) and on each time from 5～30 min decreased significantly than that before and immediate after moxibustion(P<0.05)2.Comparation of uterus time frame temperature:Compared with normal group, the difference value among each point during 5-30min and before moxibustion were significantly increased in model group, Sanyinjiao(SP6) group and Guanyuan(RN4)group(P <0.05). Compared with Sanyinjiao(SP6) group, the difference value between immediate after moxibustion and before treatment increased significantly in Guanyuan(RN4) group(P< 0.05). The difference value of each point had no significance between normal group and model group(P> 0.05)3.Comparationof gap between double sides of Sanyinjiao and Xuehai points: Compared with each other,the temperature gap between sides of Sanyinjiao and Xuehai points from 5-30 min during control group model group Sanyinjiao group and Guanyuan group have no significantly difference (P>0.05).4.Comparation of gap between double sides of Sanyinjiao-Xuehai lines:Compared with control group, the temperature gap between sides of Sanyinjiao-Xuehai lines significantly increased in model group 5-30 min after moxibustion (PO.05).30min after moxibustion, the temperature of right Sanyinjiao significantly decreased in other three groups (P<0.01). Compared with model group, the temperature gap between double sides of Sanyinjiao-Xuehai lines significantly decreased after 5-30 min in Sanyinjiao group (P<0.05), while Sanyinjiao-Xuehai lines significantly decreased after 10-30 min in Guanyuan group (P<0.05). Comparison between two moxibustion groups, the influence of Sanyinjiao group to temperature gap between double sides of Sanyinjiao-Xuehai lines was earlier than Guanyuan group (P<0.05).5.LTB4 amount of uterus:IN LTB4 amount of uterus test, the difference of all groups without statistically significant (P>0.05).Conclusion:1.Temperature of uterine body surface could significantly persist for 30 min in cold stagnation syndrome of dysmenorrhea rats and the moxibustion can make the temperature drop point in advance, making this temperature drop significantly increase. And the immediate effects of moxibustion local and distal points were different, indicating that moxibustion could promote uterine body surface infrared temperature drop during a certain period time.The therapeutic effect of moxibustion on dysmenorrhea does not depend on increasing uterine body surface temperature directly and the specific effect mechanism has to be further discussed.2.Moxbustion can decrease the temperature gap between double sides of Sanyinjiao-Xuehai lines and ease the imbalance. And the influence of moxbustion SP6 to temperature gap between double sides of Sanyinjiao-Xuehai lines is earlier than moxibustion RN4. |
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