The Establishment Of Rat Nephrotic Syndrome Induced By Mercury Poisoning And Its Accumulation Damage

Objective: It is necessary to establish the steady rat model of nephrotic syndrome induced by mercury intoxication so that we can investigate and verify the new treatment by it.Methods: I. We investigated applicable injection time and dosage for establishment of the rat model by abdominal subcutaneous injection of mercuric chloride.1.Animal grouping: 24 adult male Brown-Norway rats were divided into four groups randomly according to the different mercuric chloride dosage( 0.5mg/kg group, 1mg/kg group,2mg/kg group)and the saline control group,each group had 6 rats;2. Model Preparation: the rats in model groups were performed in the presence or absence of mercuric chloride every other day.3. Observing indexes:Monitored the body weight changes 、survival time、mental status、urine protein、blood biochemical indicators 、mercury pontent、pathological section after infected in 7、14、21、28、35 days,then we get the proof of applicable dosage and time. We perform the experiment repeatedly to verify reliability and feasibility of the model.Ⅱ.After identify 1mg/kg was the right dose to establish the model,we observe the accumulation of mercury content in the kidney. 1. Animal grouping: 24 health male BN rats, were divided into 4 equal groups,each group of six randomly according to body weight; 2.methods: Each model group of rats understood equal abdominal subcutaneous injection of mercuric chloride solution(1mg/kg)once in two days while another six BN rats was injected 0.9% sodium chloride solution as control.Each 6 rats of model group and 2 rats of control group put to death,taken out the kidney to monitor the mercury content in days 15、22、36,in order to observe the accumulation of mercury content in the kidney;3.Observing indexes:Observed BN rats mental status and monitored the mercury content in kidney.Results: Ⅰ.1.Compared with the BN rats in control group,there was appeared high proteinuria、low albumin、hyperlipidemia and edema in different degree of three model groups in days 14,the related symptoms are most prominent with 1mg/kg dose group.2. Different degree of pathological damage was observed in the kidney of rats injected with mercuric chloride solution compared with the rats in control group. The typical pathological types with nephrotic syndrome called focal segmental glomerular sclerosis under light microscope was observed with 1mg/kg dose group. It implied that 1 mg/kg dose group is suitable to establish the rat model. 3.Mercury content in blood 、urine showed the mercury content was higher during the period of infection and there was obvious dose- effect relationship. Mercury levels in blood and urinary drops rapidly after stopped the injection, but there was still a statistically significant difference compared with control group(P<0.05).Ⅱ.Mercury content measured in the kidney showed mercury excretion was drained quickly afer stopped the injection,but still persisted for a period of time and prompted mercury accumulation in kidney tissues.Conclusion: Our data demonstrated that we establish a reliable model of nephrotic syndrome induced by mercury intoxication in BN rats,which had been injected subcutaneously with 1mg/kg mercuric chloride solution for two weeks.It can provide a stable and scientific animal models for subsequent research.