Research On Trace Elements In Urinary Calculi Of Patients With Damp-Heat Constitution And Yin Asthenia Constitution In Guangdong Province

Research objectiveTo carry out comparatively study on trace elements in urinary calculi of patients with Yin Deficiency Constitution and Damp-Heat Constitution using XRD and LA-ICPMS.Research methodsAccording to previous research, we conducted a questionnaire survey to identify patients’constitution using the standard in TCM Constitution Questionnaire and Classification and Determination of TCM. A total of 53 cases, 48 valid questionnaires were collected,12 cases of Damp-Heat Constitution and 13 cases of Yin Deficiency Constitution have been identified. Urinary calculi stones of the patients who have wet-heat constitution were collected to carry out the study. Mineral structure of the stones were determined using X-ray Diffraction, trace element contents have been obtained using Laser Ablation ICPMS. The outcome were to compare with some overseas studies.ResultsMineral structure of the urinary stones collected from patients with wet-heat constitution are as follow:calcium oxalate monohydrate (CaC2O2 H20,COM), calcium oxalate dihydrate (CaC202·2H20, COD). LA-ICPMS analysis results indicate that contents of Zn, Mn, Sr, Pb and Cr increase dramatically in the stones.The data of 25 cases have (P<0.05) when comparing to data from J. Kuta, which shows the difference was statistically significant. While the damp heat group and yin deficiency group the contents of trace elements were analyzed, the test results of P values are greater than 0.05, the difference was not statistically significant. It can be considered the two groups of stones in the trace element content has no obvious difference.ConclusionMain components of 25 cases of urinary calculi collected from damp heat and yin deficiency constitutions in Guangdong area are:calcium oxalate monohydrate, two water calcium oxalate and magnesium phosphate.12 cases of damp heat and 13 cases of yin deficiency urinary calculi in Guangdong region have no obvious abnormalities. Although the damp heat and yin deficiency patients with different constitution, but there is a tendency that the enrichment of trace elements is similar, which is cause by no different of living environment and diet.Zinc content in urinary stones is nearly the same with that of J. Kuta reference value, strontium content increased obviously, which is proved by J. Kuta theory, zinc and strontium are both higher in calcium phosphorus stones. Zinc, calcium and phosphorus are easily deposited in the organic stones with the increase component of zinc. Strontium has competitive effect on calcium, by affecting the metabolism and the formation of calcium oxalate.High content of lead and chromium in stone may be related to the living environment and the toxic metal contact, it also indicates the formation of urinary stones may be caused by lead and chromium pollution in Guangdong.Copper enrichment can be in the form of carbonate composite, the reason may be related to organic carbon and calcium ion concentration; iron increased significantly but its inhibitory effect on calcium oxalate iron is not clear.Contents of manganese, nickel, cobalt and other elements increased, the mechanism is unknown, but may be related to the regional environment and food.