The Effect And Mechanism Of Enteral Nutrition At Different Time On The Nutrition Of Rats With Severe Acute Pancreatit

Objective To explore the effect and mechanism of early enteral nutrition on thenutrition of rats with severe acute pancreatitis.Methods75healthy male Sprague-Dawley rats (230~270g weight) were randomlyallocated into three groups:the sham operation group(15cases),the parenteralnutrition group(15cases),the enteral nutrition group(45cases).The enteral nutritiongroup were randomly divided into3groups by the attack time of enteral nutrition (15cases):EN-1group,EN-2group and EN-3group,received enteral nutrition in thepostoperative first day, third day,fifth day.Build SAP model by antidromic injected3.5%sodium taurocholate in the pancreatic duct.Kill rats in the three time points:thesecond day,the fourth day,the sixth day(each group of5),collect blood serum to textamylase,albumin,transferrin,total protein. assay was restored at-80℃for ELISA andIL-10and TNF-α.Pancreas and small intestine tissue were fixed in4%formalin,observe pathological change.Results About amylase,TNF-α,IL-10,after the model PN and EN group serum levelsof every index compared with the S group in all time points were significantlyhigher(P＜0.05).Compared with PN and EN-3group,the EN-1and EN-2group islower(P＜0.05),the amylase and TNF-αin EN-3group is lower than PN group in thesixth day(P＜0.05).About albumin,transferrin,total protein,after the model PN and ENgroup serum levels of every index compared with the S group in all time points weresignificantly lower(P＜0.05).Compared with PN and EN-3group,the EN-1and EN-2group is lower(P＜0.05),the albumin and total protein in EN-3group is lower thanPN group in the sixth day(P＜0.05).About pathological change,intestinal mucosa ofEN group is more thick than PN group,the villi is more higher,the sooner the better in EN group.Conclusion1.The enteral nutrition can control inflammation better,can improve state of an illnessin severe acute pancreatitis,more earlier more better.2.The enteral nutrition can improve nutrition of the rats with severe acute pancreatitis.3.The enteral nutrition can protect intestinal mucosa,is favor of intestinal functionrecovery and alimentation.