| This dissertation is composed of three chapters. The first chapter discussed the antioxidant activity in vitro and hepatoprotective effect in vivo of Compound Lobelia. The second chapter studied the effects of Compound Ainsliaea fragrans and its disassemble recipes on antioxidant in vitro and hepatoprotective effect in vivo. The third chapter summarized the study progress of Compound Ainsliaea fragrans.Chapter I Antioxidant activity in vitro and hepatoprotective effect in vivo of Compound Lobelia1. The antioxidant activity of different extracts of Compound Lobelia were estimated by the methods of measuring the DPPH radical scavenging, ABTS radical scavenging, ferric reducing antioxidant power (FRAP). The study discovered that DPPH free radical scavenging activity of ethanol extract was higher than that of water extract. ABTS free radical scavenging activity of ethanol extract (IC50=29.26±0.49μg/mL) was stronger than that of water extract (IC50==42.09±2.44μg/mL), but they were lower than that of BHT as positive control (IC50=2.47±0.09μg/mL). The ferric reducing antioxidant power of ethanol extract was higher than that of water extract, they were lower than that of BHT. The results indicated that water extract and ethanol extract of compound Lobelia had a certain antioxidant effects in vitro. However, the antioxidant activity of ethanol extract was higher than that of water extract.2. To investigate the protective effect on carbon tetrachloride (CCl4)-induced acute liver injury in mice of different extracts from Compound Lobelia, biochemical parameters were measured, biochemical parameters which include glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) in serum, superoxide dismutase (SOD) and malondialdehyde (MDA) in liver tissue homogenate. Results showed that water extract and ethanol extract could significantly reduced GOT activity and GPT activity in serum, reduced significantly the content of MDA and significantly increased SOD activity in the mice liver tissue homogenate. Further explanation water extract and ethanol extract of compound lobelia had a certain hepatoprotective effects in vivo, and the mechanism of protecting was concerned with the antioxidant capacity of liver cells. There were no related literature reports, and compound Lobelia was expected to be used to develop prevention and treatment of liver diseases. Chapter II Antioxidant activity in vitro and hepatoprotective effect of Compound Ainsliaea fragrans and its disassemble recipesThis chapter includes two parts. The first part, antioxidant activity in vitro and hepatoprotective effect of Compound A. fragrans were investigated. The experimental results found that DPPH free radical scavenging activity of ethanol extract was higher than that of water-extract and alcohol-precipitate extract, however, both of them were lower than that of positive control, which were BHT, PG and BHA. ABTS free radical scavenging activity of ethanol extract (IC50=17.12±0.52μg/mL) was stronger than that of water-extract and alcohol-precipitate extract (ICso=42.01±4.74μg/mL), but they were lower than that of positive control, which were BHT (IC50=4.39±0.17μg/mL), PG (IC50=0.98±0.04μg/mL) and BHA (ICS0=2.86±0.06μg/mL). The ferric reducing antioxidant power of ethanol extract (FRAP=1086.67±125.06μmol/g) was higher than that of water-extract and alcohol-precipitate extract (FRAP=616.67±95.77μmol/g). The study of the protective effects on carbon tetrachloride (CCl4)-induced acute liver injury in mice of different extracts from Compound A. fragrans indicated that the level of GOT and GPT were significantly decreased for ethanol extract and water-extract and alcohol-precipitate extract, respectively, and ethanol extract and water-extract and alcohol-precipitate extract had an obvious effect on reducing the content of MDA and rising the level of SOD in different degrees. The results indicated that ethanol extract and alcohol-precipitate extract of compound A. fragrans had antioxidant in vitro and hepatoprotective effects in vivo in varying degrees, and hepatoprotective effects was closely related to antioxidant.The second part, in the course of researching on the antioxidant in vitro of Compound A. fragrans and its disassemble recipes. We found that the capacity for scavenging DPPH free radical of extract of A. fragrans was strongert than that of water-extract and alcohol-precipitate extract of A.fragrans in the experiment concentration range. However, the abilities of ethanol extract of A. macrocephala to scavenge DPPH free radical was very weak. A. macrocephala and water-extract and alcohol-precipitate extract of A. macrocephala to scavenge DPPH free radical was very weak. The ability of scavenging ABTS free radical of the extract of A.fragrans (IC50=12.20±0.54μg/mL) was better than water-extract and alcohol-precipitate extract of A. fragrans (IC50=16.88±0.00μg/mL). Nevertheless, the rate of the ethanol extract of A. macrocephala, and water-extract and alcohol-precipitate extract of A. macrocephala,was30.14%,14.58%respectively while the concentration was108.10μg/mL. The ferric reducing antioxidant power of ethanol extract of A. fragrans (FRAP=1371.67±104.74μmol/g) was the best among water-extract and alcohol-precipitate extract of A. fragrans (FRAP=918.33±61.86μmol/g), ethanol extract of A.macrocephala (FRAP=124.38±16.69μmol/g) and water-extract and alcohol-precipitate extract of A. macrocephala (FRAP=59.17±7.29μmol/g). Results indicated that the A. fragrans had strong antioxidant activity and it was the key element of antioxidant activity in the compound A. fragrans. Further to track the effective part of A. fragrans suggest that the DPPH and ABTS radical scavenging activity and of methanol extract was better than that of ethyl acetate extract, and the IC50of ABTS was13.83±1.24μg/mL and14.25±1.75mg/mL, respectively. The ferric reducing antioxidant power of methanol extract (FRAP=680.52±67.04μmol/g) was stronger than that of ethyl acetate extract (FRAP=328.85±66.38μmol/g). Hydroquinone had strong capacity for scavenging ABTS free radical (IC50=1.04±0.00μg/mL) and the ferric reducing antioxidant power (FRAP=11035.25±1030.21μmol/g), and the antioxidant activity of Hydroquinone was stronger than that of positive control BHT and BHA.This research showed that methanol extract and ethyl acetate extract were antioxidant active situs of A. fragrans, and methanol extract of A. fragrans, ethyl acetate extract of A. fragrans and Hydroquinone had strong antioxidant activity. Methanol extract of A. fragrans and ethyl acetate extract A. fragrans could significant decrease the level of GPT (excepting the high dose group of methanol extract) and GOT in serum and the content of MDA (excepting the low dose group of methanol extract) in the mice liver tissue homogenate, and remarkably increase the level of SOD in the mice liver tissue homogenate. The high, middle and low dose groups of Hydroquinone could observably reduce the level of GOT and the middle and low dose groups could observably reduce the level of GPT in serum, the content of MDA of high and low dose groups was markedly decreased, and the level of SOD of high dose group was observably increase. The results suggested that A. fragrans and Hydroquinone had significant protective effects on carbon tetrachloride (CCl4)-induced acute liver injury in mice. Chapter Ⅲ Research progress of Compound Ainsliaea fragransThe third chapter was a summary of the recent study situation about Compound A. fragrans, which included effective component, pharmacological action, clinical application, content determination, quality control and so on. |
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