Studies On Chemical Constituents And Bioactivities Of The Ainsliaea Latifolia (D. Don) Sch. Bip.

The genus Ainsliaea (Compositae, tribe Mutisieae), a medicinally important genus in traditional Chinese medicine, comprises about 70 species worldwide, is mostly distributed in Southeast Asia. Among them,44 species are distributed in the south regions of China. So far, several sesquiterpenes, triterpenes and flavonoids, have been isolated from this genus. Ainsliaea latifolia is a medicinal herb growing mainly in Tibet, Yunnan, Sichuan, Guizhou, Guangxi and Hainan provinces of china and has been used as a folk medicine for the treatment of rhumatism, traumatic injuries, edema, and stomach ache. However, no phytochemical and biological studies of A. latifolia have been reported. In this dissertation, we investigated the chemical constituents from A. latifolia systematically, together with anti-inflammatory and anti-tumor activities.Investigation of chemical constituents of the 80% enthanol extract of the Ainsliaea latifolia afforded fourty eight compounds by various chromatography methods (silica gel, Sephadex LH-20, preparative-HPLC, preparative-TLC), including thirteen sesquiterpenoids and its glycosides, one diterpenoid, nineteen triterpenoids, three flavones, four steroids, seven phenolic acids and one others. Among which there are twelve new compounds, other compounds were isolated from this plant for the first time.Part of compounds,1-12,14-25,27-31,45 were evaluated for cytotoxicity activity against five human tumor cell lines A549, HCT116, MCF-7, HUH7 and SMML-7721 by MTT assay. However, only compound 9,18 and 23 showed strong cytotoxicity activities against five human tumor cell lines at 50 μmol/L, while the others are weak or inactive against five human tumor cell lines.Compound 1-2,5-6,15-23 were evaluated for their COX-1 and COX-2 inhibitor activity. Among them compound 2 and 18 were the most potent ones against COX-2 with IC50 values of 3.98 and 2.31μM. Compound 16,19 and 20 showed mild activity, of which the IC50 values were at 18.94,19.48 and 31.02 μM. The other compounds showed weak or inactive activity.