| Objective:The experiment adots the initial periodontal therapy combined with low level laser threatment to treat patients with chronic periodontitis. Evaluating its therapeutic effects by observing the periodontal tissue morphology,clinical periodontal index and using enzyme-linked immunosorbent detection technologies to detect the related changes of cytokines.Preliminarily clarify low level laser on anti-inflammatory and repair reconstruction process of periodontal tissue,providing a new method of treating in the treatment of periodontitis.Methods:Selected18patients with chronic periodontitis with a total of80tooth left and right sides is symmetrical. After oral hygiene education, first, useing ultrasonic sealer choose the appropriate power and suitable water flow rateing about20～30ml/min, respectively, supragingival scaling and subgingical scaling. And then, randomly divided into two groups,the experimental group and the control group. Experimental group, laser treatment was performed by using a galliumaluminum-arsenide (GaAlAs) diode laser after root planning. The physical parameters used during the treatment were as follows:wavelength,808nm; average output,80mw and continuous wave output.Oral health education and ultrasonic cure for baseline, sulcus bleeding index (SBI), probing depth (PD), and clinical attachment level (CAL) were recorded at baseline,1,4, and12weeks after the laser treatment. Gingival crevicular fluid samples were collected from two groups,.The paper strips were inserted into the crevice until mild resistance was felt and left in place for30seconds. Strips contaminated by bleeding were discarded. Strips were placed into coded Eppendorf tubes and stored at-20℃until further enzyme processing. Basic-fibroblast growth factor levels, IL-8and IL-1β in the collected gingival crevicular fluid were measured. The data collected were analyzed using a statistical software package. Once the obtained data were normally and homogeneously distuibuted. Differences between groups and between different time points within each group were tested by the t-test and ANOVA, respectively.The level of significance was0.05.Results:All patients included in the evaluation completed the6-month study period and none missed any appointments. Healing was uneventful in all cases. No adverse effects, such as periodontal abscess or dentine hypersensitivity, related to the laser irradiation have been reported.1. At1week, for the change of SBI and PD,, only the experimental group the SBI difference had statistical significance compared with baseline (P<0.05); At4weeks, both groups were statistically significant compared with baseline (P<0.05). At12weeks, situation stays same while the experimental group makes more significant difference (P<0.01); For both groups, the SBI showed most significant improvement in patients with probing depth of more than6mm. For CAL, the experimental group showed signs of improvement (P<0.05) at4weeks; both group indicated improvement (P<0.05) at12weeks while the experimental effect more significantly (P<0.01).2. For the levels of b-FGF, the experimental group showed steady rise after treatment, while the control group rise after falling. At12weeks, the two groups showed significant difference (P<0.05).About the change of IL-1β, and the change of IL-8, the experimental group show better results than control group, while the difference between two groups was not significant (P>0.05)Conclusion:1. After initial periodontal therapy, periodontal condition improved of all periodontitis patients.2. LLLI can accelerate the healing of periodontal tissue by reducing the SBI, decreasing the PD, improving the CAL and increasing levels of b-FGF in the GCF of the patients. |
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