Effect Of Periodontal Initial Therapy On Serum Oxidative Stress Level In Chronic Periodontitis With Type 2 Diabetes Mellitus

Objective: Periodontitis has gradually taken on more importance because the fact that it is the Sixth Complication of diabetes mellitus,the research is mostly about the mechanism between diabetes mellitus and periodontitis,but the effect of periodontal initial therapy on type 2 diabetes mellitus is not well known.The aim of this research is to investigate the effect of periodontal initial therapy on serum OS level in chronic periodontitis with type 2diabetes mellitus.?Methods: Sixty-six subjects from Department of Endocrinology and Department of Periodontic from July 2017 to February 2018 were divided into three groups comprising 20 subjects in Group H(healthy controls),22 subjects in Group P(chronic peridontitis patients),and 24 subjects in Group PD(Type 2 diabetes mellitus with chronic periodontitis).sites of probing pocket depths(PPD),sites of clinical attachment loss(AL),sites of bleeding on probing(BOP)were measured both at baseline and 1.5months later after periodontal initial therapy in Group P and PD.The serum levels of AGEs,sRAGE,esRAGE,SOD,8-OHdG were performed on Groups P and PD then were recorded at baseline and again at 3 months.?Results: Significant differences were perceived in the indexes at baseline,1.5 months and3 months after PIT between the three groups using one-way ANOVA.1.It shows statistical significance that the sites of PD and BOP in Group PD are more than Group P(p<0.05);2.The sites of PD?AL and BOP in Group PD after PIT are less than before in both Group P and Group PD,it shows statistical significant difference(p<0.05);3.It shows statistical significance that the serum level of AGEs,sRAGE,esRAGE,SOD,8-OHdG in Group H,Group P and Group PD which were examined by Elisa kit(p<0.05);4.Meanwhile,the five selected parameters of serum oxidative stress respectively in Group P and Group PD were examined by Elisa kit,compared before with after the PIT,it shows statistical significance(p<0.05).Comparing the autophygosome of PMN between 3 groups byTEM,it shows that autophygosome in DP group is more than P group and H group ?Conclusion: Periodontal initial therapy(PIT)is effective in reducing serum OS level in T2 DM patients.1.In the similar local irritation cases,the inflammatory state of periodontal tissue in the PD patients with T2 DM is more severe than those without it;2.Oxidative stress level in serum of PD patients with T2 DM is more severe than those without it;3.The periodontal initial therapy not only can improve the inflammatory state of periodontal tissue,but also can lead to better control of high serum OS which is caused by chronic hyperglycemia state in T2 DM patients.Type 2 diabetes Mellitus with periodontitis: PMN has more autophagosomes than chronic periodontitis patients and healthy individuals.Autophagy is a conserved dynamic catabolic process,whicn plays a criticial charactor in preserving cellular meostasis.Although it is not clear if there is a positive or negative effect from autophagy for the cell,it can be certain that it is a signal of functional disorder in the cell.So we conclude that the PMN function of type 2 diabetes mellitus.