Clinical Significance In Gastric Cancer Diagnosis Of Combined Detection Of Serum TK1, TSGF, CEA, CA125and CA19-9

【Objective】To explore the clinical significance of combined detection of serum TK1, TSGF, CEA, CA125and CA19-9in gastric cancer.【Methods】This was a retrospective analysis of study of176inpatients from January2012to December2012in Gastrointestinal Surgery of First Affiliated Hospital of Fujian Medical University, which meet the inclusion and exclusion criteria. Among patients with pathologically confirmed116cases of gastric cancer as test group,35cases of benign gastric were used as the benign control group and25healthy subjects were used as the healthy control group. Immunoblotting enhanced chemiluminescence was used to detect serum TK1, and TSGF was measured by enzyme-linked immunosorbent assay (ELISA method), and CEA, CA125, CA19-9was detected by multiple tumor protein chip. To compare various types of tumor markers in gastric cancer test group and two control groups, and explore the relationship between various types of gastric cancer patients with serum tumor markers with sex, age, tumor differentiation, tumor stage and lymph node metastasis. ROC curves were made with various types of tumor markers by SPSS17.0statistical software, and compared the area under the ROC curve, then determine the optimal cutoff value. By comparing the area under individual ROC curves and the sensitivity and specificity, which of tumor markers of all kinds of test group and the control group and joint detection(Combined detection contain TK1), to explore the clinical significance of combined detection of serum TK1, TSGF, CEA, CA125and CA19-9in gastric cancer.【Results】(1)TK1was significantly higher in gastric cancer test group than the benign control group and the healthy control group, and differences were statistically significant (P<0.05), the difference between benign group with the control group was not statistically significant (P>0.05).(2)TK1was higher in stage Ⅳ patients than stageⅠ,Ⅱ, and the difference was statistically significant (P<0.05); TK1was higher in lymph node metastasis than those without metastasis, and the difference was statistically significant (P<0.05);(3) The largest substance under ROC curve among single tumor marker was0.795in TK1; The highest sensitivity of two joint, which was up to80.5%, was TK1+TSGF, and specificity was62.9%;The highest sensitivity of three joint, which was up to86.2%, was TK1+CEA+CA125, and specificity was85.7%; The highest sensitivity of four joint, which was up to86.2%, was TK1+TSGF+CA125+CA19-9, and specificity was82.9%; Five combined sensitivity of87.9%, and specificity was80.0%;(4) To identify the gastric benign and malignant lesions by the ROC curve, then determine the optimal cutoff value of TK1, TSGF, CEA, CA125, CA19-9, which were namely:1.7PM/L,59.3U/ml,3.3ng/ml,25.4ng/ml,27.8mU/mL;(5)The highest positive rate of detection of gastric cancer by Individual tumor markers was TK1(56.9%), others were TSGF(32.8%), CEA(30.8%), CA19-9(23.3%), CA125(13.8%).【Conclusion】(1)TK1may become one of the identification of benign and malignant lesions of the stomach may test indicators;(2) TK1should be better than TSGF, CEA, CA125, CA19-9when identifying of gastric cancer;(3) TK1has some diagnostic value at different stage and lymph node metastasis of gastric cancer;(4) The sensitive of combined detection of tumor markers was significantly higher than the individual detections; Combined sensitivity, specificity, the best joint is TK1+CEA+CA125when combined detection of gastric cancer.