The Effect And Regularity Of Dietary Factors On Blood Lipids And Lipid Peroxidation In Rats

Objectives To study the independent or joint effct and regulitary of dietary factors such as high fat diet(HFD),ethanol intake,Grape Procyanidins(GPC) and VE on blood lipids and lipid peroxidation in rats,thus offer theory basis for instructing people creat scientific diet structure.Methods Adult Wistar rats were divided randomly into 7 groups such as basal diet, high fat diet(HFD),alcohol, GPC and VE, breeded continuously for 6 weeks. Blood lipid level before and after experiment including serum triglyceride(TG), totlecholesterol(TC), high density lipoprotein-cholesterol(HDL-C),low density lipoprotein-cholesterol(LDL-C);lipid peroxidation degree including serum active oxygen, malondialdehyde(MDA), brain lipofuscin,spleen cell DNA contenUiver tissue HE staining section; antioxidantive ability including superoxide dismutase (SOD) activity, total antioxidant competence(T-AOC) were studied. Results 1.Compared with the level before experiment,serum TG,TC.plasma MDA,active oxygen increased significantly in HFD group. 2.Compared with normal control group,serum LDL-C,brain lipofusin level increased,serum HDL-C,spleen cell DNA content decreased,liver tissue damage occurred,SOD activity and T-AOC increased in HFD control group andHTD+ethanol group.3.Compared with HFD control group,serum HDL-C level,DNA content,SODactivity decreased,plasma MDA level increased,liver damage was more serere inHFD+ethanol group.4.Compared with HFD control group,serum blood lipid,plasma MDA leveldecreased,DNA content,T-AOC increased,liver damage was more minimal inHFD+GPC group.5.Compared with HFD+ethanol group,serum blood lipid,plasma MDA leveldecreased in both high and low doze GPC group.Plasma MDA leveldecreased,DNA content,SOD activity,T-AOC increased,liver tissue was similarwith normal control group in HFD+ethanol+GPC(high doze) group.6.Compared with HFD+ethanol control group,blood lipid level didn't changedsignificantly.Plasma MDA,active oxygen brain lipofusin level decreased,DNAconteat increased in HFD+ethanol+VE group.There was no difference between theantioxydant activety of GPC and VE of same doze.Conclusion l.HFD can increase blood lipid level,aggravate lipid peroxidationdegree and decrease antioxidant ability.2.Ethanol intake following HFD can aggravate lipid peroxidation degree.3. GPC can decrease blood lipid level and inhibit lipid peroxidation caused byHFD or HFD+ethanol and showed a doze-effect relation.4.VE can inhibit lipid peroxidation and has no effect on blood lipid level.