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Effects Of Dietary Fiber From Citrus Peel On Blood Lipid-decreasing In Rats (Rattus Norvejicus)

Posted on:2010-11-19Degree:MasterType:Thesis
Country:ChinaCandidate:J LiFull Text:PDF
GTID:2144360278479627Subject:Food Science
Abstract/Summary:PDF Full Text Request
Dietary fiber (DF) is a mixture including cellulose, hemicellulose and lignin, pectin and soluble polysaccharide, and other components. As an extremely important nutrient, dietary fiber has gained the concern of the nutritionist at home and abroad. In the development of functional food, dietary fiber has been payed more and more attention. Dietary fiber, a good resource of dietary fibers, is the by-product of citrus processing. But it hasn't been made good use of so far. The recent researches indicate that such cardiovascular diseases as hyperlipidemia, arteriosclerosis and coronary heart disease etc. are concerned with deficiency of dietary fiber in food. So it is necessary to develop safe, functional food of high quality dietary fiber. In this article, the effects of dietary fiber from Citrus sinensis Osbeck cv. Jincheng which extracted by enzymatic on hyperlipidemia SD rats by exmined their lipid metabolism and lipid peroxidation. In order to understand the role of citrus peel dietary fiber played on the area of preventing atherosclerosis (AS). This study is of supply theories about developing safe food from Citrus residues which contains dietary fibers of high quantity.To randomly divide SD rats into 5 groups based on body weight, according to the dosage of the DF given, they are normal control group (group NC), high fat control group (group FC), low level of DF group (group L), middle level of DF group (group M) and high level of DF group (group H). Besides group NC, the others were given a lipid-rich diet containing 10% yolk powder, 0.5% cholate, 5% lard and basic diet in order to induce hyperlipidemia. The DF intervention groups were given 2.5%, 5% and 10% dietary fiber in addition. After feeding DF 4 weeks, blood and tissue specimens were collected, and bodyweight of rats, food intake of rats, serum TC, TG, HDL-C, LDL-C, SOD, MDA, GSH-Px,organ-body ratio, visceral fat weight fecal weight and fat were determined. The histology of liver was observed. A significance analysis of difference by SPSS11.5 showed that: Compared with group FC, the level of bodyweight and food intake of rats were decreased appreciably(P>0.05), all DF intervention groups can lower TC, TG, LDL-C and elevate HDL-C, especially for group H can markedly reduce TC and LDL-C(P<0.01) and group M also can markedly reduce TC(P<0.01).Compared with group FC, all DF intervention groups can markedly elevate SOD, GSH-Px activities(P<0.05 or P<0.01) and lower the level of MDA appreciably(P>0.05). Compared with group FC, the organ-body ratio and visceral fat weight in all DF intervention groups were decreased appreciably (P<0.05 or P>0.05). Compared with group FC, the fecal weight and fat contents in all DF intervention groups were significantly higher than that in group FC(P<0.05 or P<0.01). Livers in all DF intervention groups had fatty degeneration to some extent and the group FC was the most serious, which was in correspondence with the lipids levels.Through the comparison of blood lipid level on rats, citrus dietary fiber can decrease the serum TC, LDL-C of rats and increased the serum HDL-C of rats. The addition of dietary fiber has a linear relation with the blood lipid level. In addition, the lipid comprehensive index (LDL-C/HDL-C, TG/HDL-C, AI) decreases with increasing of the addition of dietary fiber. It shows that citrus dietary fiber has a mitigative effect on abnormal lipid metabolism of rats. The paper provides excellent theoretic foundation and experiment proof about comprehensive utilization of citrus.
Keywords/Search Tags:Dietary fiber, Hyperlipidemia Cardiovascular disease, Lipid metabolism, Lipid peroxidation
PDF Full Text Request
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