| In this study, lactic acid bacteria, yeast, Bacillus subtilis mixed solid state fermentation method, using plastic film bags full price of moldy feed fermentation process to produce the amount of lactic acid as index. By optimizing the test combination, determine the optimum fermentation conditions; using enzyme-linked immunosorbent assay kit before and after the fermentation of aflatoxin B1 (AFB1), ochratoxin A (OTA), deoxynivalenol (DON), corn red enone (ZEN) four levels of toxins; finally determined by the effect of feeding trials add fermented moldy complete feed production performance of weaned piglets, pigs fed on the ability to evaluate.Test one:Research on the full price of feed fermentation process parameters mildew mixed bacteria.. Lactic acid bacteria were used, mixed bacteria (lactobacillus: Yeast:Bacillus subtilis== 3%:1%:3%) fermented moldy full price of feed, the first single bacteria and compare the effect of mixed fermentation, followed by mixed fermentation trials using four factors and four levels orthogonal design of different fermentation time, temperature, feed water ratio, pH effect on the yield of lactic acid. The results showed that:mixed fermentation better results than single fermentation, fermentation products AFB1 lower levels; and mixed fermentation lactic acid content in the group improved by 19.9% than single set of fermentation, lactic acid content of 3.26%; moldy feed AFB1 content between lactic acid production and a negative correlation. Mixed fermentation combination of optimum conditions: fermentation temperature 35 ℃, fermentation time 3d, water ratio 1:0.6, pH value of 7.Test two:Combine with the best fermentation parameters, explore traits of fermentation products. Under optimum conditions (ie, the proportion of mixed inoculum of lactic acid bacteria:Yeast:Bacillus subtilis= 3%:1%:3%, mixed inoculation time 27h, fermentation temperature 35 ℃, fermentation time 3d, the ratio of water 1:0.6, pH= 7) moldy complete feed fermentation, during fermentation, the fermentation time was measured periodically sampled after fermentation product characteristics, including the viable cell number and pH changes during the fermentation, after fermentation nutrient mycotoxins and the change before and after the fermentation, the parallel test was repeated three times and averaged. The results showed that the group mixed fermentation pH decreased significantly, which mixed fermentation pH at 24h after the group showed a significant downward trend, reaching 4.30 at 72h of fermentation, significantly lower than the control (P<0.05); viable count of lactic acid bacteria in the fermentation increased rapidly during the rapid proliferation in the inoculated lactic acid fermentation, to 9.861ogCFU/g or more, which is consistent with the change in pH of fermentation products, because of a large amount of lactic acid produced in the rapid proliferation of acids, so that rapid pH decline. Yeast growth changes relatively mild,3d within reach peak growth, and then gradually declined. Bacillus subtilis rapid proliferation in the fermentation, the fermentation 3d around the maximum, and then decreased rapidly; the end of fermentation, fungal content from 5.31 logCFU/g dropped to 2.92 logCFU/g. When fermentation 3d, compared to the control group, the test group was significantly decreased number of viable cells mildew (p<0.05); nutrient content will not change much, feed crude protein content and P content increased slightly, by 6.1% and increased 12.3%; after fermentation AFB1 decreased from 22.46 ug/kg to 4.32 ug/kg, several other mycotoxins levels also have different degrees of decline, have reached the state allows the content range.Test three:The effects on weaning performance and apparent digestibility of raw moldy complete feed by mixed fermentation. Full price of feed substitute fermentation fermentation conditions above moldy feed as part of the full price of feeding diets, the choice to select 28-day-old weaned 60 were randomly assigned into two principle groups of three repeats, each repeat 10 piglets fed in the same column. Control group was fed a normal full price of feed, the test group fed complete feed plus the mixed fermentation of moldy feed (1:1), the pre-feeding period of seven days, a trial period of 31 days. The results showed that feed intake and daily gain of the test group than the control group were significantly improved, from fecal microbial detection results, the test group lactic acid content of 8.791ogCFU/g, E. coli only 4.621ogCFU/g, while the control group than the number of lactic acid bacteria fermented material fed to low, and has significantly increased the number of E. coli, diarrhea first few test group less than the control group, the experimental group decreased by 60% compared to the control group rate of diarrhea. |
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