Animal Model Development And Evaluation Of Functional Diarrhea Spleen Deficiency Syndrome

Functional diarrhea is a common clinical functional gastrointestinal disease, its clinical manifestations is similar with the syndrome of spleen deficiency which the main performance is diarrhea, and in the clinical, functional diarrhea often see with spleen deficiency syndrome, so the combination of disease and syndrome is important to study function of diarrhea. Functional diarrhea of spleen deficiency syndrome of clinical studies, and laboratory research is less, so the establishment of functional diarrhea of spleen deficiency animal model is necessary. This article is from the theoretical and experimental study and to put forward modeling methods and evaluation methods to verify functional diarrhea of spleen deficiency syndrome.Theoretical studyObjective:To put forward and evaluation method for making functional diarrhea spleen deficiency animal model method. Method:Reading the literature about functional diarrhea and spleen deficiency syndrome, the functional diarrhea animal model making method and evaluation method, and test, verify the idea is feasible, and the form and perfect production and evaluation method offunctional diarrhea of spleen deficiency syndrome animal model. Results:Access to functional diarrhea related literature, that the current functional diarrhea animal model making methods are insufficient, evaluation methods also existdefects. Spleen deficiency animal model making methods are various, the existence of the animal model of different, varying lengths of time, evaluation model of complex. Based on the spleen deficiency combined with diarrhea from disease, the lack of animal model. Therefore, in the consult massive literature situation, we put forward basic principles of making functional diarrhea spleen deficiency animal model for:characteristics of functional diarrhea and spleen deficiency syndrome animal model with the etiology, symptoms, the course of the disease. The symptoms, clinical manifestations of functional diarrhea of spleen deficiencymust be both functional diarrhea with spleen deficiency syndrome. The animal model should have at least75%bowel soft (paste) or sample, animal body weight increased slowly, loss of appetite, weight loss, less dynamic, limb weakness, hairdry wait for a symptom. Functional diarrhea with spleen deficiency syndrome of relatively long duration, to human and animal life length ratio requirement modeling time should be in the modeling, the animal model of human disease and functional diarrhea "diagnosis of symptoms for at least6months, nearly3monthssymptoms consistent with the above criteria" time in the same proportion. The basic requirements of functional diarrhea spleen deficiency animal model for the evaluation of:1, the etiology, symptoms, duration, in line with the characteristics of functional diarrhea with spleen deficiency syndrome;2, the reference indexes of clinical research possible to detect animal model;3, the clinical effective drugscounterevidence to model, effective drugs. The cause of diarrhea of unknownfunct ion, it can refer to spleen deficiency syndrome of TCM etiology in making animal model. Conclusion:Over fatigue and improper diet is the most common cause of clinical spleen deficiency syndrome, so we can choose a foreign classic chronic diarrhea model-high lactose diet (improper diet) on traditional Chinese medicine diagnosis system withBeijing University of Chinese Medicineof water environment of small PingTai Railway Station legislation (overstrain) model, both with TCM et iology and requirements, requirements symptoms can achievefunctional diarrhea with spleen deficiency syndrome, the14day of modeling, but also can satisfy the functional diarrhea of spleen deficiency longer courserequirements.Experimental studyObjective:To verify the fabrication method of functionaldiarrhea of spleen deficiency animal model and evaluation method. Methods:In this experiment, we will study is divided into two parts, respectively, copy andselected drug model, functional diarrhea of spleen deficiency animal model is more suitable to. In the first part of the experiment, we used the male Wistar rats aged three weeks135, method of making copy of common spleen deficiencyoverstrain and chronic diarrhea, divided into blank control group, swimming group, water environment, water environment platform standing group swimming platform standing group, high lactose, high lactose lactose group with swimming group, high lactose water environment of small platform standing group5h, high lactose water environment of small platform standing group9h group, high lactose water environment of small platform standing group of14h group, model14days, and every day was recorded on the model, the calculation of diarrhea index. Fifteenth days, each group of10rats randomly selected from the blood from the abdominal aorta in rats, the removal of the appl ied factor, basic formula feed, animal was recorded on the day, calculate the diarrhea index. Twenty-ninth days, the remaining rats, with the former method. In the second part of the experiment, we use classical medicine Shenlingbai zhu powder in treatment of diarrhea of spleen deficiency model to disprove. The male Wistarrats aged three weeks90,divided into blank control group, model group, high dose group (referred to as SHENLINGBAI SHU reference group, high dose group)(referred to as there ference of Shenlingbaizhu powder SHENLINGBAISHU group), low dose group (referred to as the low group), berberine group (referred to as the western medicine group), using the selected in the first part of the experiment of highlactose water environment of small platformmodel14day stand9h method, and then the blank control group, model group, pure water by gavage, other groups of rats were administered with different doses of the drug, a total of7days. Twenty-second days to complete the sampling, sampling methods such asbefore. Results:The symptoms were recorded and measured, we found14days after modeling, high lactose and small platform standing9h rats loose stools, diarrhea, anorexia, weight increased slowly move less, hair loose not Zesymptoms. Diarrhea index higher than the blank control group, compared with the blank control group P<0.01; decrease the serumamylase activity, compared with the blank control group P<0.01; elevated serum lactic acid content, compared with the blank control group P<0.01. Twenty-eighth, high lactose and smallplatform to stand on the rats of group9h still Shenpi fatigue, hair loose, amylase activity was lower than control group (P<0.01), so we think the high lactose water environment of small platform standing9h is compared with the animal model of functional diarrhea of spleen deficiency making method. In the second part of theexperiment, after treatment, Shenling Baizhu powder in high, low dose group, canimprove symptoms of rats, rats stool normal or slightly soft, shiny hair, body weight growth speed, increase food intake, and high group and the serum amylase activity participation in rats in the blank control group, no significant difference; ginseng, ginseng high low group and lactic acid content in the blank control group close, no significant difference; ginseng, ginseng in the high grouprat urinary D-excretion rate than the control group significantly increased (P<0.01). Therefore it can be considered that the Shenlingbaizhu powder has therapeutic effect on9h rats on high lactose water environment of small platform. Conclusion:high lactose water environment of small platform standing9h is making method of functional diarrhea spleen deficiency animal model of excellent. Evaluation of functional diarrhea of spleen deficiency should be based on symptoms, objective index (lactic acid, serum amylase, urine D-xylose)method by.