CD4 ~ +, CD8 ~ + Experimental Study On Effect Of Sijunzitang Peripheral Blood And Spleen Deficiency

Objective：To explore the effect of si junzi decoction on the CD4+,CD8+contentin the peripheral blood in spleen deficiency syndrome rats.Methods：One hundred and two experimental SD rats weighted （200±20）g ofSPF were randomly divided into four groups, twenty-four rats in normal controlgroup,twenty-six rats in spleen deficiency syndrome model group,naturalrecovery group and si junzi decoction group respectively.Making modelfactors:Using composite factors.In addition to normal control group, the otherthree groups were made spleen deficiency syndrome models.Using three compositefactors of exhausting Qi and dissipating stagnant Qi drugs,undue hunger andovereating,physical overstrain to make spleen deficiency syndrome models.Usingxiao chengqi decoction (1.5ml/100g) to intragastric administration every otherday and fasting on that day,then providing sufficient amount of eating andswimming to the endurance limit the other day.Intervention factors:Rats innormal control group were normal fed and regular intragastric administrationwith physiological saline solution (1.5ml/100g) seven weeks every other day.Ratsin spleen deficiency syndrome model group were fed the same way as the makingmodel method seven weeks.Rats in natural recovery group were fed the same wayas the spleen deficiency syndrome model group the first four weeks,then changedto the regular feed the last three weeks.Rats in si junzi decoction group werefed the same way as the spleen deficiency syndrome model group the first fourweeks,then changed to the regular feed and intragastric administration with sijunzi decoction (1.5ml/100g) once every day the last three weeks.Results：1.General case(1)Normal control group: activity was normal,sensitive response,hair was luster,defecate was normal,weight gained,appetite was normal.(2)Spleen deficiency syndrome model group:activity was abnormal, lassitude,cluster,squinting,extrados,hair turned withered and yellow thenfalled off,loose stool, weight loss, poor appetite.(3)Natural recovery group and si junzi decoction group:the first four weeks,manifestation was the same as spleen deficiency syndrome model group,the lastthree weeks, manifestation was the same as normal control group.2. WeightThe first four weeks, there was statistically significant difference betweenspleen deficiency syndrome model group, natural recovery group,si junzidecoction group and normal control group （P<0.01，P<0.05）.The last three weeks,there was statistically significant difference between natural recoverygroup,si junzi decoction group and spleen deficiency syndrome model group（P<0.01，P<0.05）.3. AppetiteThe first four weeks, there was statistically significant difference betweenspleen deficiency syndrome model group, natural recovery group,si junzidecoction group and normal control group （P<0.01）.The last three weeks, therewas statistically significant difference between natural recovery group,sijunzi decoction group and spleen deficiency syndrome model group（P<0.01）.4. Swimming time,diameter of the tail, anal temperatureSwimming time: There was no statistically significant difference between them(P>0.05).Diameter of the tail: There was statistically significant differencebetween spleen deficiency syndrome model group and normal control group（P<0.05）.There was statistically significant difference between si junzi decoction groupand spleen deficiency syndrome model group（P<0.01）. Anal temperature: Therewas no statistically significant difference between them (P>0.05).5. The spleen index and The thymus indexThere was no statistically significant difference between them (P>0.05).6. CD4+,CD8+content in the peripheral blood CD4+: There was statistically significant difference between natural recoverygroup,si junzi decoction group and normal control group（P<0.01）. There wasstatistically significant difference between natural recovery group,si junzidecoction group and spleen deficiency syndrome model group（P<0.05）.CD8+: Therewas no statistically significant difference between them (P>0.05).CD4+/CD8+:There was no statistically significant difference between them (P>0.05).7. Manifestation in microscopeSpleen deficiency syndrome model group: Mucosal defect, angiectasis hyperemiaof lamina propria.Natural recovery group: Angiectasis hyperemia andinflammation of lamina propria was not obvious,infiltration of inflammatorycells was visible.Si junzi decoction group: Angiectasis hyperemia of laminapropria.Normal control group: Almost normal.Conclusions：1.Spleen deficiency could reduce defense mechanism of the gastric mucosa,thenlead to functional decrease of gastric mucosa barrier. The local immuneregulation of CD4+,CD8+content in the peripheral blood reflexed the defensemechanism of gastric mucosa barrier,also reflexed the whole immune regulation.2.Spleen deficiency could lead to functional decrease of gastric mucosabarrier,it might be one of pathophysiological basis of chronic gastric disease.3.Si junzi decoction group as the treating drug could repair the gastric mucosaby improving CD4+,CD8+content in the peripheral blood,adjusting the whole immunefunction by improving the local immune regulation,then strengthened thedefense function,it might be one of the mechanism of treating spleen deficiencysyndrome,also reflexed the mechanism of repair the gastric mucosa usinginvigorating spleen and replenishing qi drug.