Spleen Metastasis Of Gastric Cancer Mouse Model And The Gene Expression Profiles

Objective:To study the relationship of Spleen Deficiency and gastric carcinoma,and to probe into method of constructing metamouse model of gastric carcinoma and Spleen Deficiency,which combines with disease and syndrome. Then to reveal the change of gene expression of the model.Methods:Established NIH and BALB/c-nu/nu mice model of Spleen Deficiency by using vinegar. Second,inducted gastric carcinoma on NIH mice for studying the relationship of Spleen Deficiency and gastric carcinoma by using DENA,and transplanted tissues of fresh human gastric carcinoma to the stomach wall of BALB/c-nu/nu mice of Spleen Deficiency by surgical orthotopic transplantation,then,examined the difference of gene expression of the model by using the technology of gene expression chip.Results:Successful established model of Spleen Deficiency and gastric carcinoma and metamouse model of gastric carcinoma and Spleen Deficiency;observed mat Spleen Deficiency is the foundation of gastric carcinoma;observed the obvious difference of gene expression,which involve in differentiation,apoptosis,proliferation,material metabolism of gastric carcinomatous cell,between SGC-7901 and gastric carcinomatous cells of this model.Conclusions:Using method explained in this article can establish stable model of Spleen Deficiency and gastric carcinoma and metamouse model of gastric carcinoma and Spleen Deficiency,which all combine with disease and syndrome;proved that Spleen Deficiency mainly promotes occurrence and development of gastric carcinoma,which is realized by disordering correlative gene expression of gastric carcinoma.