| Abstract:Objective:"Zheng shi san qi san" is an experience formula of traumatology invented by CTM’s late famous Professor Zheng Huaixian who is a professor of orthopedics and traumatology of traditional Chinese medicine and martial artist. The experiment was studied to understand the toxicity and mechanism of Zheng shi san qi san through gavaging the rats with the medicine in order to provide experimental basis for the safety of sports injury and clinical application of orthopedics.Methods:This experiment drew lessons from Chinese herbal medicine serum a pharmacology.75SD rats were randomly divided into5groups.4drug groups were gavaged according to the drug doses conversion between human and animal, twice per-day lasting21d; the control group were gavaged0.9%N.S as same as doses, also once per-day lasting21d. Then the blood of each rat was collected for separating blood serum to examine ALT and AST, the liver tissues were selected to weigh and observe the degree of their injury by microscopes. The differences of liver’s toxicity and mechanism of the Zheng shi san qi san and Liao yuan qi li san were discussed in order to offer the safe evidence for clinical application in this procedure. The experimental data was dealt with the software of statistics.Results:(1) General observations:taking medicine3weeks, the rats" appearance. behavior, activity, response of stimulation, state of mind, appetition, fece, urine, back wool, skin color, nose, eyes, mouse had no abnormalities. The weight of rats in each group increased. All the rats had no poisoning symptom.(2) The examination result of ALT and AST showed the differences of the value was not significant.(3) System dissection with taking medicine21days, pathological section indicate that there are not pathological change of swelling, hyperemia,dropsy.emphysema in the rats" liver.(4) After taking medicine3weeks, the differences of the rats’ weight and index of the liver in groups A, B, C, D. E were not significant.Conclusions:(1) The doses of each group of Zheng shi san qi san and the comparison group of Liao yuan qi li san were safe.(2) The study showed that the results of cellular toxicology of liver cell of each group of Zheng shi san qi san and the comparison group of Liao yuan qi li san were safe. |
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