Pharmacognosy Studies Of Herba Lophatheri

Herba Lophatheri, the dry stemleaf of Lophatherum gracile Brongn,belongs to thefamily of Gramineae, it has been used for a fever, the bacteriostasis, the diuresis function intraditional Chinese medicine. The present paper has conducted the foundational researches,including following parts:1. Books on Chinese medicine textually research with the literature summary. Thesummary includes the domestic and foreign literature, introduce the modern applicationresearch progress about Herba Lophatheri in recent years.2. Carried on the pharmacognosy about raw material for medicine.3. Study on the quality specifications ofHerba Lophatheri, Using the ultravioletspectrophotometric method, taking rutin as a standard, has carried on the total flavanonecontent determination. Finally, tests 8 habitats which collects, 11 raid of Lophatherumgracile Brongn total flavanone content between10.0～16.5mg/g, Zhejiang productionthe total flavanone content to be highest.4. Carries on the qualitativequota to the Herba Lophatheri chemicalcomposition research, differently withdrew the spot to carry on the system pre-experiment, and carried on Herba Lophatheri the bacteriostasis experiment, determinedbacteriostasis effective spot was Methyl alcohol extraction, discovered the backwoods coli,the pseudomonas aeruginosa, the golden yellow staphylococcus, the second grade chaincoccus has the bacteriostasis effect, the bacteriostasis density is 1:10. Established thin layerdistinction method of Herba Lophatheri. Using ICP-MS technology to conducted to thetrace element content Herba Lophatheri, summarized the difference trace element of eachplace. According to the literature reported the utilization known ingredient vitexin took therepresenting material, has conducted the fingerprint atlas appraisal research. UsingLC-MS/MS technology for a total chromatographic fingerprint research, tentativelyidentified six known chemical composition.5. To optimise extraction process.The extraction of total flavonoids was choosen as theassessment index. The optimum ethanol extraction process was selected with the orthogonaldesign. Results: The optimum ethanol extracting process conditions follow as: adding 18fold of 75％ethanol, extracting for 1.0h and one in all, ethanol dosage is predominantinfluencing factor. 6. Formulated the Herba Lophatheri quality specification draft and The draft explains.