| Objective: Treatment of burn wound healing in rats and Research Yulangsan(YLS) aqueous extract of the aqueous extract Yulangsan main pharmacodynamicstudies, using a wide range of basic experimental methods in accordance with therats and small experiments on mice as a positive control drug, designed Yulangsanaqueous extract of burn wound healing, anti-inflammatory and analgesic effects,antimicrobial effect, the mechanism of acute toxicity, skin irritation and otherexperiments.Methods:(1) SD rat burn wound model was prepared:Experiments using160SD rats were fixed in the supine position on makingtouch wood, shaving with electric clippers rat dorsal spine on both sides of the hairshaved clean, can not damage the skin, to the hair scope of the right and left,each about4cm×4cm after using depilatory15%sodium sulfide to finish off theback of the hairs, and then rinsed with clean water back part of the site of injury:rat back hair removal zone,10to12seconds of injury time, visual observation ofthe wound after the applicator cases, continuous applicator and observation for20days, daily wound area measurements with a vernier caliper, scarring and woundhealing observed rate of the case.(2) KM mouse experimental model was prepared:2.1on the anti-inflammatory effects, with80KM mice were two methodsinflammatory experiment: 2.1.1experiment on mice ear swelling:50KM mice were divided into fivegroups, the control group, positive control group and dexamethasone5mg kg-1and divided into three groups medication aqueous extract high YLS100g crudedrug dose group kg-1, YLS water extract crude drug dose80g kg-1, YLSaqueous extract low dose50g crude drug kg-1, administered orally three days in arow according to100%xylene test methods, and then the mice were killed bycervical dislocation, cut along the baseline ear ears, weighing, poor mouse ears atthe same weight of said film swelling, swelling inhibition rate was calculated.2.1.2paw swelling in mice experiments:50KM mice were divided into fivegroups, the control group, positive control group and dexamethasone5mg kg-1and divided into three groups medication YLS aqueous extract high doses groups100g crude drug kg-1, YLS water extract dose80g crude drug kg-1, YLSaqueous extract low-dose group50g crude drug kg-1, continuous gavage threedays. according to1%carrageenan the method tests, mice were killed by cervicaldislocation after, after clipping the feet equal parts, weighed to the differencebetween the full weight of the mice were about the same degree of swelling,swelling inhibition rate calculation.2.2on the analgesic effect, with40KM mice, the mice were made to touch ontwo planks fixed, fixed-made touch supine position on the board, the mice withelectric clippers shaving hair shaved back of the spine, Do not damage the skin, tothe hair range left and right, each about3cm×3cm, after using depilatory8%sodium sulfide to finish off the back of the hairs, then rinse the back part, dividedinto three groups Yulangsan extract groups (high, in low doses) and salinecontrol group, three groups YLS medication applicator2times a day, each timethe applicator is9am,5pm applied to three days in a row last day,30minutes afterthe applicator, each group of mice intraperitoneal injection of0.7%acetic acid,each injection of0.2ml. writhing and immediately observed and recorded the timeand within15minutes the number of bodies writhing reactions.2.3In the acute toxicity test, with20KM mice, hair removal, an area of about3cmx3cm, after using depilatory8%sodium sulfide to finish off the back of thehairs, then rinse the back part. Extraction on Yulangsan group after back hairremoval area coated with0.5ml YLS drug applicator4times a day, each time the applicator is9am,11am,3pm,5pm, continuously applied to14days. Mice wereobserved daily and body weight measurements recorded.2.4Experimental allergic skin irritation with70KM mice were twoexperimental methods2.4.1skin allergy test with30mice, said the right back hair, an area of about3cmx3cm, after using depilatory8%sodium sulfide to finish off the hairs on theback of the right, and then rinse the back part of the mice were divided into3group and the saline control group (A),0.5%2,4dinitrochlorobenzene positivegroup (group B), YLS aqueous extract group (group C), continuous applicatorseven days, the7th day of the experiment, Similarly applicator right and left back,left-back group B coated with0.1%2,4dinitrochlorobenzene concentrations.After the4th applicator1h, washed with water back on all drugs in mice. Instantlyand after observation and observation time is divided into12h,24h,48h,72h againobserved an allergic skin reaction conditions.2.4.2Skin irritation test in mice with40mice, hair removal back area is dividedinto two experiments, a single applicator and multiple applicators-A single applicator with20experimental mice were divided into two groups,YLS water extract group and the saline control group, on the YLS extracted groupof mice painted with0.5ml YLS extract hair removal area, after using the gauze,to prevent the animal licking,6h after washing with water in addition to visuallyobserve and record the drug-coated parts of erythema and edema after the drugtime to observe divided30min,24h,48h and72h after.-Multiple applicator with20mice divided into two groups, YLS water extractgroup and the saline control group, the applicator in the same way, a continuouscoating seven days, once a day, every six hours to maintain contact and stop drugobserve three days, recording applicator site erythema and edema, and so on.2.5In vitro antibacterial liquid medicine in the steam autoclave eight poundsafter20minutes as the experimental liquid. Staphylococcus aureus, Pseudomonasaeruginosa, Escherichia coli, Candida albicans were inoculated agar slant,37°Cfor24hours, the culture broth was inoculated sterile37°C for18hours and theninoculated with sterile broth medium37°C for6hours, diluted with sterile physiological saline before use to1:1000. germ-tube10, the first tube broth plus1.5ml, add1ml of culture broth remaining tube, the first test tube add the liquidafter mixing, of1-9contain fold dilution of liquid. That1:4,1:8,1:16,1:32,1:64,1:128,1:256,1:512,1:1024Article1,2,3,4,5,6,7,8,100.05mlbacteria added to each tube pipe section9without bacteria, mix37°C for24hours, a sterile nutrient agar plate sub-species of medium37°C for24hours.Results:1. YLS aqueous extract of burn wounds have significant therapeutic effect,applicator for20consecutive days, especially on the difference between the controlgroup (P <0.05or P <0.01). YLS aqueous extract divided into high-dose,medium-dose and low-dose have a significant effect, the healing rate is relativelyhigh P <0.01(high and medium dose) or P <0.05low doses. YLS water extractinto0.1ml/day,0.2ml/day,0.3ml/day,0.4ml/day,0.5ml/day applicatoralso has the effect of treatment, the healing rate of the better P <0.05or P <0.01.2.YLS aqueous extract of a significant anti-inflammatory effect, results in pawswelling in mice ear edema in mice experiments and YLS aqueous extract high,medium and low dose could significantly inhibit mouse ear inflammation, pawswelling (P <0.05).3. YLS aqueous extract in mice writhing test in a significant analgesic effect onthe effective analgesia in mice over time up to2h.4. YLS water extract in the acute toxicity tests have obvious differences, thesituation did not appear abnormal mice after treatment, normal weight for thecontrol group P>0.05.5. YLS aqueous extract in skin allergies and irritation experiments, YLSaqueous extract high, medium and low dose groups were not there and irritatingsymptoms associated with allergies, normal weight mice, not unusualperformance.6. YLS Water Extract on Staphylococcus aureus minimum inhibitoryconcentration (MIC)1:64, Escherichia coli and Pseudomonas aeruginosa (MIC) of1:4, no inhibitory effect on Candida albicans. The results showed that: YLS water extract has anti-inflammatory, analgesic,antibacterial, anti-infective, improve wound tissue structure, promote woundhealing and other pharmacological effects. |
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