The Effect Of Chaibanliuhe Decoction On GAS, MTL And SS In Rat Models With Chronic Renal Failure

Objective: To observe the effect of Chaibanliuhe decoction on the levels of Gastric secrete element(GAS), Gastric dynamic element(MTL), somatostatin(SS) in rat chronic renal failure(CRF) model, and to explore its mechanism.Methods: Eighty male wistar rats were pathogen-free lw.Sixteen rats were randomly selected as control group and the rest64rats were used to establish the CRF models by the method of5/6nephrectomy. After the CRF models were established, the64rats were divided into4groups (model control group, high Chaibanliuhe decoction group, low Chaibanliuhe decoction group and Niaoduqing control group) randomly with each group of16rats.at the end of the fourth week after the operation the levels of Scr and BUN in serum were detected to estimate whether the CRF models were established successfully.Then All rats were administered with the method of oral gavage.High and low Chaibanliuhe decoction group were administered with Chaibanliuhe decoction2ml [crude drug4.34g/(kg.d) and2.17g/(kg.d)]. Niaoduqing control group were administered with niaoduqing granules2ml [crude drug2.0g/(kg.d)]. Control group and model group were administered with2ml normal saline.and the gavage continued for8w.Then The levels of Scr, BUN, GAS, MTL and SS in serum were detected, and the pathological changes of Gastric mucosa and kidney were observed by hematoxylin-eosin (HE) staining.Results:Compared with model control group, Scr, BUN, GAS and MTL decreased while SS increased significantly in every administration group; The levels of Scr, BUN, GAS and MTL in the high Chaibanliuhe decoction group were lower than that in the low Chaibanliuhe decoction group and Niaoduqing control group while the level of SS was higher; Compared with that in the Niaoduqing control group, GAS and MTL decreased while SS increased in the low Chaibanliuhe decoction group; There was no significant difference between low Chaibanliuhe decoction group and Niaoduqing control group with the levels of Scr and BUN. Conclusion:Chaibanliuhe decoction can improve the gastrointestinal symptoms of CRF in rat model by decreasing the levels of GAS and MTL and increasing the level of SS.