The Research On The Effect Of Liuwei Dihuang Pill On The Susceptibility Genes CLCF1of Postmenopausal Osteoporosis With Kidney Yin Deficiency

Objective:In the early stage of the research topic, on the basis of the use of liuwei dihuang pill treatment of postmenopausal osteoporosis, kidney Yin deficiency syndrome, and applies the RT-PCR technique to detect treatment, before and after treatment CLCF1, JAK-STAT signal pathway JAK1and STAT4and CBP mRNA expression level, compare the differences before and after, and combined with TCM symptoms curative effect, clarify the POP kidney Yin deficiency syndrome and CLCF1mediated JAK-STAT signaling pathways regulate the correlation of CBP.Methods:Randomly chosen POP30cases of kidney Yin deficiency syndrome subjects, questionnaire to survey the subjects lifestyle and health, etc., with dual energy X-ray absorptiometry (dexa) detection is a lumbar spine (L2-4) and on the left side of the femoral neck, greater trochanter and Ward’s area bone mineral density; Application of liuwei dihuang pill treatment, treatment for3months, before and after treatment were detected using RT-PCR technique CLCF1, JAK-STAT signal pathway JAK1and STAT4and CBP mRNA expression level, and compare the differences before and after, and connecting with the curative effect of TCM score before and after the treatment, to clarify the POP kidney Yin deficiency syndrome and CLCF1mediated JAK-STA letter T pathways regulate the correlation of CBP.Results:Randomly chosen POP kidney Yin deficiency syndrome subjects were30cases, fall off in1case, a total of29cases.Probability of TCM symptoms curative effect analysis of9cases had marked effect,31.03%;18patients effectively,62.07%;Invalid in2cases,6.90%;The total effective rate93.10%.POP kidney Yin deficiency syndrome patients before treatment CLCF1 expression (0.0208±0.0112) expression is (0.3282±0.7789) after treatment significantly increase, more significant difference (P<0.05).POP kidney Yin deficiency syndrome patients before treatment JAK1expression (0.0471±0.0225) expression is (0.0220±.0201) after treatment significantly up and down, with significant difference (P<0.01).POP kidney Yin deficiency syndrome patients before treatment STAT4(0.1745±0.1434) expression is (0.1018±0.0702) decreased significantly after treatment, the more significant difference (P<0.01).POP kidney Yin deficiency syndrome patients before treatment JAK1(0.1281±0.1673) expression is (0.0577±0.0467) decreased significantly after treatment, the more significant difference (P<0.05).Conclusions:By rt-pcr technique comparison before and after treatment and JAK CLCF1gene STAT signaling pathways in JAK1and STAT4and CBP mRNA expression level expression situation, combined with clinical syndromes curative effect analysis, we think: POP kidney Yin deficiency syndrome is associated with CLCF1, mechanism of action of liuwei dihuang pill to treat POP kidney Yin deficiency syndrome may be related to regulating the CLCF1mRNA expression level and mediated JAK-STAT signaling pathways regulating downstream CBP.