| Objective:This article is to study the Dispase I which induced proliferative vitreoretinopath of the rabbit model, Preliminary discuss the Huyusanjie tablet on the mechanism of inhibiting Muller cell proliferation in proliferative vitreoretinopathy model,take proliferative vitreoretinopath which with cell proliferation as the common pathological features for the representative eye, aimed from different angles to explore the effect and mechanism of Huyusanjie tablet in the treatment of the same type of eye disease. Provide a guidance for clinical, also provide new ideas and methods of Chinese herbs in the future prevention and treatment of proliferative vitreoretinopath.Methods:(1)Establish Dispase I induced rabbit proliferative vitreoretinopathy model, using Fundus photography technology and Immunohistochemistry, observing Huyusanjie tablet in Rabbit model in retina morphology changes after the intervention;(2) use Immunohistochemistry to explore Huyusanjie tablet tablet on Dispase-induced the rabbit model GFAP and VIM expression changes of Muller cells;(3) by the use of vitro cell culture technology to culture Muller cells, and to combine with serum pharmacology method to study Huayusanjie tablet in preventing experimental PVR model.Results:(1) Develop the PVR animal model:blank control group comparedwith model control group in hyperplasia series hype is statistically significant (P<0.01).(2) After treatment7、14、21and28days, model control group compared with positive control group,Huyusanjie tablet HD control groups and TD control groups in hyperplasia series is statistically significant (P<0.01).After treatment7and14days, model control group compared with Huyusanjie tablet LD control groups in hyperplasia series is statistically significant (P<0.01),but is not statistically significant(P>0.05) after treatment21and28days.(3) There were GFAP expression in every groups except blank control group, The IOD value of GFAP of model control group was higher than the control group blank, there was statistically significant (P<0.01); Model control group compared with positive control group, there was statistically significant (P<0.05), compared with Huyusanjie tablet HD control groups and TD control groups, there was statistically significant (P<0.01), but compared with LD control groups there were not statistically significant (P>0.05).(4) There were VIM expression in every groups, The IOD value of VIM of model control group was higher than the control group blank; there was statistically significant (P<0.01); Model control group compared with Huyusanjie tablet HD control group, there was statistically significant (P<0.05), compared with positive control group and Huyusanjie tablet TD control groups, there was a significant difference, but compared with LD control groups there were not statistically significant (P>0.05).(5)High-dose of Huayusanjie Tablet compared with blank control group is statistically significant (P<0.01); dauno-micin group and middle-dosen low-dose group compared with blank control group is not statistically significant (P>0.05).High-dose of Huayusanjie Tablet could inhibit proliferation of cultured Muller cell in vitro, while middle-dose group and dauno-micin group showed lower inhibitory effect on Muller cell.Conclusion:(1)Dipase I can independently initiate the development of PVR without the addition of exogenous cells and cytokine and has a high success rate.(2) Huyusanjie tablet can reduce the expression Levels of GFAP and vimetin of PVR rabit reduced by Dipase I.(3) Huyusanjie tablet can directly inhibit the proliferation of Muller cell. |
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