Study Of The Effect And Mechanism Of Removing Stasis Tablets In Preventing Experimental Proliferative Vitreoretinopathy

BACKGROUND: The Huayusanjie Tablet is used as a therapeutic option for animals with experimental PVR . The aim of this study was to investigate the effect and mechanism of Huayusanjie Tablet on the experimental PVR in vitro and in vivo. METHODS: Autologous whole blood was injected into the vitreous cavity of albino rabbits after canalization,thus induced experimental PVR model. Concentration of PDGF .in vitreous cavity was measured by ELISA method, and the expression of ICAM-1 and VCAM-1 was observed by immunohistochemicai examination. RPE proliferation was measured by MTT assay.RESULTS: There was preventive and curative effect of Huayusanjie Tablet on. experimental PVR in vitro and in vivo. (1) Result of fundus and pathology-microscope examination showed the average stage of PVR in the eyes treated with Huayusanjie Tablet (9.6g/Kg or 2.4 g/Kg) was significantly lower than that in the control group on days 28(P<0.01),and there was significant difference between the high-dose group and low-dose group(p<0.05).However , the effect of Huayusanjie Tablet was obviously lower than daumomicin(P<0.01). (2) Huayusanjie Tablet could increase the number of ganglion cell in retina(P<0.05 or P<0.01). (3) The concentration of PDGF in the vitreous of Huayusanjie Tablet -treated eyes was significantly lower than in the control group. And daumomicin was also lower than Chinese medicine. (4) Expression of ICAM-1 and VCAM-1 were observed on proliferative membranes and vascular endothelial cell. Huayusanjie Tablet could decrease the expression of two cellular adhesion molecule described above, but there was no difference within Huayusanjie Tablet groups and daunomicin group. (5) High-dose of Huayusanjie Tablet could inhibit proliferation of cultured RPE cell in vitro,while low-dose group and daunomicin group(80ng/ml) showed lower inhibitory effect on RPE.CONCLUSION: The data suggest that Huayusanjie Tablet can prevent the formation and development of PVR in' vitro- and in.vivo.Its mechanism concern with factors described below:lowering the concentration of PDGF,decreasing the expression of ICAM-1 and VCAM-1 in proliferative membrane and vascular endothelial cefl,directly inhibiting proliferation of RPE. in vitro.