Study On The Lifestyle Factors Of Blood Stasis And Caloric Form Of Endometrorrhagia Caused By IUD-wearing And Effects Of Gongning Granules On The Expression Of Uterine VSMC Of Caspase-3, Caspase-9

Objective:The purpose of this project is to IUD bleeding side effects patients livingfactors of clinical research,and on the Preliminary studies basis of“Study on the ProtectingEffect and Mechanism of Mai-Su Powder on Vascular Endothelial function of DAO inRabbits”， researchers use Non-invasive method to build IUD contraceptive ratmodel.Through the expression of the observation uterine vascular smooth muscle cellsCaspases-3, Caspases-9,we explore the uterine proliferation of VSMC apoptosisregulation pathways,and preliminarily explore endometrial the inflammation betweenIUD insertion and uterine vascular remodeling relations,and further clear blood stasis andclearing away heat, the prevention and control mechanism of the the hemostatic Tiaojinglaw.Method:1.Clinical Research:A cross-sectional survey method, select outpatient cases per unitfor the survey, each node certificate in accordance with homemade Stagnant Heat IUDbleeding in patients with adverse clinical questionnaire (see Appendix), and collection ofpatient-related lifestyle factors.Medical statistical analysis of the collected data.2.The modeling evaluation: Using a non-invasive method based on the rat estrouscycle (IUD) placed after vaginal-cervical and abdominal method (intraperitoneal-uterine placed IUD), IUD contraception rat model. Comprehensive evaluation of theindicators, providing clinical IUD contraceptive match mature animal model suitable forIUD bleeding side effects study.3.Experimental study: Scanning electron microscopy uterus VSMC ultrastructural changes, using immunohistochemical methods, system detects the uterus the the VSMCThe Caspases3and the the Caspases9expression analysis after IUD insertion uterineproliferation of VSMC apoptosis and vascular remodeling to explore Gongning particlesmechanism.Results:1.Clinical research: Statistical analysis, the difference was statistically significant atthe10life: the high incidence of the age of30-34years old, manual workers more, thelevel of education in high school and above level IUD side effects of bleeding patients,personality and more impatient or worried, partial addicted to diet heavier motherhood≥3times, the number of operations of uterine surgery≥3times, a previous history ofgynecological inflammation, the majority opposed metal IUD, set IUD3-5year afterabnormal uterine.2.The modeling evaluation: Non-invasive method IUD contraceptive rats modelingmethod of observation.3.Experimental study:Particles of Gongning can significantly improve theultrastructural changes of the the IUD contraceptive rat uterus VSMC,while increasinguterine immunohistochemical expression of VSMC.Conclusion:The side effects of the IUD bleeding patients affected by factors of life is obvious.Greatly extent of the non-invasive method the IUD contraceptive rat model simulatingclinical IUD bleeding incidence of side effects process.Gongning particles significantlyincreased the uterus VSMC caspase-3and caspase-9expression.