Comments On The Non-incision Modeling Method Of Rat IUD And Research On Regulation Of Gongning Granules On The IUD Remodeling Mechanism Of Uterine Vessels

Purpose: To evaluate the non-incision modeling method of rat IUD and explore theregulation of Gongning granules on the remodeling mechanism of uterine vessels.Methods:Method I: Use SD female rat as the experimental subject. Randomly divide50ratsinto5groups: non-incision model group, abdomen-incision model group, non-incision fakeoperation model group, abdomen-incision fake operation group and normal control group.Make the IUD non-incision model, IUD abdomen-incision model, IUD non-incision fakeoperation model group and IUD abdomen-incision fake operation model and incise theabdomen of the rats to observe the successful placing rate, falling rate and effectivecontraception rate and rate of IUD inflammation.Method II: Use SD female rat as the experimental subject. Randomly divide50ratsinto5groups as the method I and duplicate IUD modeling method also as described in themethod I. Test the concentration of the tumor necrosis factor-α（TNF-α）, transforminggrowth factorβ（TGF-β）and intercellular adhesion molecule-1（ICAM-1） of theperitoneal flushing fluid of the five groups by enzyme linked immunosorbent assay. Thenby the same method, test the concentration of thromboxane （TXB2）, metabolite ofthromboxane （TXA2）, concentration of6-keto-PGF_1α, metabolite of prostaglandin（PGI2）and concentration of plasminogen activator （t-PA） as well as the concentration ofuterine tissue homogenate, alkaline phosphatase （AKP） and carbonic anhydrase（CA）.Method III: Use SD female rats as the experimental subject. Divide50rats into5groups at random: model group, fake operation group, Gongning granula group,indomethacin group and normal control group. Duplicate the IUD non-incision model and IUD non-incision fake operation model. Test the expression of the surface indicator,α-SM-action of vascular smooth muscle cell （VSMC） and proliferating cell nuclearantigen （PCNA）by Immunohistochemical S-P method to calculate the proliferating rateof uterine vascular smooth muscle cell（VSMC）（PCNA/α-SM-action positive cells*100%）.Result:Result I: The successful IUD placing rate of non-incision model group andabdomen-incision model group is90%and80%respectively and the falling rate ofnon-incision model group and abdomen-incision model group is0%and10%respectively.The contraception rate of both groups is100%and the inflammation rate of non-incisionmodel group is10%, that of abdomen-incision model group is40%and that ofabdomen-incision fake operation group is30%.Result II:1. The concentration of the rat Peritoneal flushing liquid TNF-αofnon-incision model group is apparently lower than that of abdomen-incision model groupwith significant difference（P<0.01）.2. The concentration of the rat peritoneal flushingfluid of non-incision model group is apparently lower than that of abdomen-incision modelgroup with significant difference（P<0.01）.3. The concentration of non-incision modelgroup is close to that of abdomen-incision model group without significant difference（P>0.05）.4. The concentration of rat peritoneal flushing liquid TXB2of non-incisionmodel group is apparently higher than that of abdomen-incision model group withsignificant difference（P<0.05）.5.The concentration of the rat uterine cavity lavage fluid,6-keto-PGF_1α of non-incision model group is apparently lower than that ofabdomen-incision model group with significant difference（P<0.05）.6. The concentrationof the rat uterine cavity lavage fluid, t-PA of non-incision model group is apparently lowerthan that of abdomen-incision model group with significant difference（P<0.01）.7. Thenrate of6-keto-PGF1a/TXB2of rat uterine cavity lavage fluid of non-incision model isapparently lower than that of abdomen-incision model group with significant difference（P<0.01）.8. The concentration of the rat uterine tissue homogenate,AKP of non-incisionmodel group is close to that of abdomen-incision model group without significantdifference（P>0.05）.9. The CA concentration of rat uterine cavity tissue homogenate ofnon-incision model group is close to that of abdomen-incision model group without significant difference（P>0.05）.Result III:1. The indicator of VSMC of model group, α-SM-action decreasessignificantly compared with that of normal control group; After treatment, the expressionof α-SM-action of all the groups has increased obviously and extremely obviously. Theexpression of α‐SM‐action of Gongning granules group increases apparentlycompared with that of model group. It is close to that of normal control group withoutsignificant difference（P>0.05）. Although the α‐SM‐action expression of indomethacingroup increases apparently but it still significantly differs from that of Gongning Granules.2. The expression of PCNA of VSMC of model group increases apparently compared withthat of normal control group. After treatment, the PCNA expression of all the groups hasdecreased apparently. The expression of Gongning granules decreases apparentlycompared with that of indomethacin group with significant difference（P<0.05）, whichindicates that the expression of Gongning granules group is closer to that of normal controlgroup than that of indomethacin group.3. The rate of PCNA/a-SM-action of rat VSMC ofmodel group increases apparently compared with normal control group. After treatment,the rate of PCNA/a-SM-action has decreased apparently. The appreciation rate ofGongning granules differs significantly from that of indomethacin group（P<0.01）, whichindicates that the appreciation rate of Gongning granules is closer to that of normal controlgroup than that of indomethacin group.Conclusion:Conclusion I: By observing visually and comparing the rats of non-incision modelgroup and that of abdomen-incision model group, it is found that the inflammation of therats of abdomen-incision model group is apparently more serious than that of non-incisionmodel group. It can be concluded by analysis that the abdomen-incision model group has aslightly lower successfully IUD placing rate than non-incision model group withoutsignificant difference （P>0.05）However, the IUD falling rate of abdomen-incision modelgroup is slightly higher than that of non-incision model group without significantdifference（P>0.05） but the inflammation rate of abdomen-incision model group isapparently higher than that of non-incision model group with significant difference（P<0.05）. Therefore, the non-incision IUD model is better than the abdomen-incisionIUD model.Conclusion II: The significant changes of the various indicants of peritoneal flushing liquid （TNF-α、TGF-β、ICAM-1），Uterine cavity lavage liquid(TXB2、6-keto-PGF_1α、t-PA)and uterine tissue homogenate（AKP、CA）demonstrate that the abdomen-incisionmodel group has a higher inflammation rate than non-incision model group with seriousinjury to uterine. Thus the abdomen-incision model group is more likely to cause IUDnegative reaction.The various index of non-incision model group and abdomen-incision model groupare analyzed by comparing with each other in Experiment I and Experiment II atmacro-level and micro-level respectively. It can be seen from Experiment I andExperiment II that the IUD non-incision modeling is better than abdomen-incisionmodeling and non-incision modeling is the ideal IUD method for rats.Conclusion III: The Gongning granula with the effect of removing stasis and clearingheat and menstruation-regulating helps transfer VSMC from synthetic phenotype（pathologic）into contractile phenotype（physiologic） by relieving the morphologicaldamage of micro-structure and ultromicor-structure of endometrium VSMC andregulating the expression of VSMC、α-SM-action、 PCNA so that the impairedendometrium vascular changes from pathologic remodeling to adaptive remodeling toeliminate the abnormal angioarchitecture of uterine vascular so as to achieve the purposeof remodeling of uterine vascular and preventing and curing blooding after IUD placement.Furthermore, the effect of Gongning granule in curing IUD blooding is more obvious thanthat of indomethacine.