| Objective：To determine the content of different areas and different productionenterprises of hawthorn, coke Hawthorn granules,In order to provide scientific dataand basis for the quality evaluation system of hawthorn, hawthorn and coke hawthorngranules.Methods: Using thin-layer chromatography to Qualitative identification the effectivecomponents inHawthorn Hyperoside,by single factor and orthogonal test on ultrasonicextraction of hawthorn flavonoids to choose the optimization process,and hyperin asindex was determined the content of total flavonoids in Hawthorn method by UVspectrophotometry,and the contents of5kinds of flavonoids determination ofhawthorn and formula granules were detemined by HPLC.Results: TLC qualitative identification results:11Pieces of hawthorn samplescontain Hyperoside ingredients. Granules of different enterprises have less obviousHyperoside spots, experimental hawthorn granules numbered1-3and granules ofnumbered4-6have less obvious spots. granules No.8-11Hawthorn and cokehawthorn, the spot is obvious.To test the Flavonoids content of11Pieces Origin Hawthorn by UVspectrophotometry and the results in the order:Ningxia (6.430%)> Shandong (6.404%)> Yunnan (6.335%)> Henan (6.120%)>Qinghai (5.919%)> Hebei (5.505%)> Beijing (5.152%)> Guangxi (5.054%)>Zhejiang (4.660%)> Hubei (4.549%)> Hunan (4.182%).To test three different companies Hawthorn Flavonoids granules by UVspectrophotometry and the order: number K of YiFang, Guangdong (3.437%)>number G of Jiang Yin Tianjiang (2.947%)> number A of Shenzhen Sanjiu(2.855%)> number H of Jiangyin tianJiang (2.665%)> number C of ShenzhenSanjiu (2.486%)>numbers I of Jiang Yin Tianjiang (2.452%)> number E of Shenzhen Sanjiu (2.135%)> number D of Shenzhen Sanjiu (2.045%)> number F ofShenzhen Sanjiu(1.919%)>number B of Shenzhen Sanjiu (1.480%)>NO J of JiangYin Tianjiang (1.260%).To determinate five pieces hawthorn flavonoids content of11origin samplesby HPLC and the concentration in each sample in order:Vitexin rhamnoside: Shandong (0.524%)> Yunnan (0.414%)> Beijing(0.291%)> Qinghai (0.203%)> Hubei (0.193%)> Henan (0.126%)> Hebei (0.100%)>Guangxi, Zhejiang, Ningxia, Hunan origin were not detectedVitexin: Yunnan (0.322%)> Shandong (0.284%)> Beijing (0.203%)> Hebei(0.190%)> Qinghai (0.154%)> Henan (0.111%)> Guangxi (0.101%)> Zhejiang(0.090%)> Hubei (0.056%)> Ningxia (0.031%)> Hunan not detected;Rutin: Shandong (0.590%)> Yunnan (0.240%)> Qinghai (0.232%)> Beijing(0.180%)> Hebei (0.157%)> Henan (0.072%)> Zhejiang (0.066%)> Guangxi(0.056%)> Hubei, Hunan, Ningxia not detectedHyperoside: Yunnan (2.087%)> Hebei (1.998%)> Shandong, Qinghai(1.917%)> Ningxia (1.844%)> Henan (1.494%)> Hunan (1.389%)> Zhejiang(1.354%)> Beijing (1.156%)> Hubei (0.819%)> Guangxi (0.478%)Quercetin: Shandong (1.066%)> Yunnan (0.335%)> Qinghai (0.288%)> Henan(0.183%)> Hebei (0.156%)> Zhejiang (0.120%)> Hubei (0.113%)> Beijing(0.103%)> Guangxi (0.094%)> Ningxia (0.088%)> Hunan UndetectedTo determinate five kinds hawthorn flavonoids content of three differentcompanies by HPLC and the concentration in each sample in order:Vitexin rhamnoside: sanjiu F number (0.266%)> sanjiu D number (0.140%)>Jiangyin INumber (0.120%)> sanjiu E number (0.116%)> sanjiu A number (0.101%)>Jiangyin H number (0.067%)> sanjiu C number (0.065%)> Jiangyin G Code(0.057%)> party K Number (0.040%)> Jiangyin J (0.034%)> sanjiu B Number(0.021%)Vitexin: Yifang K number (0.346%)> sanjiu A number (0.278%)> sanjiu Dnumber (0.062%)>Jiangyin G number (0.059%)> sanjiu E number (0.052%)> sanjiuC number (0.043%)> Jiangyin H number (0.035%)> sanjiu F number (0.032%)>Jiangyin J NO (0.031%)> Jiangyin I, sanjiu B number was not detected;Rutin: Rutin: sanjiu D number (0.208%)> sanjiu A, Jiangyin J number(0.096%)> sanjiu C seriesNumber (0.047%)> sanjiu E number (0.041%)> JiangyinH number (0.035%)> Jiangyin G number (0.028%)> Jiangyin I numbers (0.020%)> sanjiu F number (0.010%)> one K Sanjiu B No. not Detected.Hyperoside: Jiangyin H number (0.479%)> Jiangyin G number (0.035%)> Knumber Yifang (0.268%)> sanjiu B number (0.216%)> Jiangyin J NO (0.200%)>sanjiu F number (0.125%)> sanjiu D number (0.098%)> sanjiu A number (0.026%)>sanjiu E number (0.022%)> Jiangyin I numbers (0.016%)> sanjiu C number was notdetectedQuercetin: Jiangyin G number (0.262%)> Jiangyin I numbers (0.227%)> Knumber Yifang (0.211%)>sanjiu B number (0.208%)> sanjiu A number (0.205%)>sanjiu F number (0.178%)> Jiangyin J NO (0.141%)> sanjiu E number (0.131%)>sanjiu D numbers (0.102%)> sanjiu C number (0.098%)> Jiangyin H number(0.021%).Conclution: Conclusion: UV experimental results show that Flavonoids isdifferent between different areas. The content of Ningxia, Shandong, Yunnan ishigher. Hawthorn Granule content differences between each sample, the Yifang,Guangdong has the highest content of total flavonoids.HPLC results showed that different areas hawthorn, especially formulations ofdifferent enterprises in the sample， five kinds of flavonoids are quite different.11Pieces of hawthorn samples, the content of Vitexin rhamnoside, rutin,quercetin ingredient is highest in Shandong, following by Yunnan; the content ofVitexin, hyperoside is highest in Yunnan,following by Shandong.11batches hawthorn granules of three different enterprises, the vitexinrhamnoside content is highest in Shenzhen Sanjiu F; the Vitexin content is highest inGuangdong Yifang K; the rutin content is highest in Shenzhen Sanjiu D; theHyperoside content is highest in Jiangyin H; the quercetin content is highest in GJiangyin.In short, the subject carried out and the results obtained, in order to provide atheoretical basis and data support for the hawthorn quality evaluation. |
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