Studies On The Measure Of Deter Mination The Content In The Main Components In Hawthorn With HPLC

As an important kind of medicinal and edible plant resource, hawthorn covers awide area of planting and cultivation in China which yields a great amount.Pharmacologic studies indicate that hawthorn contains many different kinds ofphysiologically active ingredients, such as proanth ocyanidins, flavone, phenolic acid,triterpenic acid, organic acid, etc. This dissertation established a set of analysismethods of these active ingredients by HPLC, which is adopted to analyze the majoractive ingredients in hawthorn including hawthorn acid，ursolic acid， oleanolic acidchlorogenic acid， epicatechin, proanth ocyanidins B2, B5, C1, hyperoside,isoquercitrin, etc. The HPLC system consists of two P230II high-pressure constantflow pump, UV230II variable wavelength UV detector and EC2006chromatographicworkstation, produced by Dalian Elite Co., Ltd.The measuring chromatographic conditions for triterpenic acid: chromatographiccolumn: Thermo Hypersil C18ODS (250mrn×4.6mm,5μm); mobile phase:acetonitrile-methanol-water (60:25:15); flow velocity:0.8mL·min-1; detectionwavelength:210nm; column temperature:30℃; sample size:10μL. Relatedcoefficient of regression equation of hawthorn acid, ursolic acid and oleanolic acidare0.9996，0.9998and0.9997respectively; linear range:0.051~0.256μg,0.082~0.431μg,0.022~0.106μg; recovery rate:1.09%,0.96%,1.08%. The measuringchromatographic conditions for flavone, proanth ocyanidins, phenolic acid:chromatographic column: Thermo Hypersil C18ODS(250mrn×4.6mm，5μm);mobile phase: A:500μL/L phosphoric acid aqueous solution; B: methanol/acetonitrile=30:70, including500μL/L phosphoric acid; the gradient elutioncondition:(min/B%)0/14,8/14,15/18,25/18,36/50,40/50,40/14: detectionwavelength:280nm(0～8min),340nm,(8-36min):280nm(36～40min); flowvelocity:0.8mL·min-1; temperature:30℃; sample size:10uL. The experimental results show that the separation effects of active ingredientsare preferable; the related coefficient of regression equation, namely r, is0.9945～0.9968; the relative standard deviation is RSD<5.0%,; and the recovery rate is 88.96%～107.14%. These methods are simple, rapid and accurate, and can be usedfor quality control and analysis measurement of hawthorn crude herb, extracts, andpreparations.These analysis results indicated that ursolic acid was high in the substances oftriterpenic acid in hawthorn; phenolic acid substances mainly contained hyperin andisoquercitin; high contents of proanth ocyanidins could be regarded as representativeingredients of active substances in hawthorn; citric acid was high in organic acidsubstances. The first innovation point of this dissertation lay in the accuracy,precision and sensitivity of this method in addition to the achievement of hawthornacid, ursolic acid and oleanolic acid. The second was that active ingredients appearedat the biggest absorptive wavelength by variable wavelength analysis so that theanalysis achieved the best results.