| Purpose:This topic passes to observe the influence that the Chinese manipulativetherapy hurts the nerve in the week blood outside the domestic rabbit to pass theorganization pathology of quality and harm part to the impatient soft organization andanalyze the impatient soft organization of tuina treatment hurts of town pain mechanism,and Chinese manipulative therapy exertion town pain function time regulation, the aim isfurther guiding tuina clinical practice, raise the clinical treatment level that the impatientsoft organization hurts.Method:Taking a big ear white experiment rabbit is30, random choose20adoptionsheavy the thing hang empty sink into to pound at the impatient soft organization of methodestablishment hurts to test rabbit animal model and is divided into the tuina treatment set,model matched control10for each according to the random number form,10restexperiment rabbits establish for the blank matched control.The tuina treatment set gives atuina skill treatment, once|d,15mins|time, cure in a row3ds;Blank matched control andmodel matched control not to any special handle, only set against to shine on anobservation.After building the mold24hs, for the1st time the30mins,90mins and150mins of treatment empress, for the third time the30mins,90mins and150mins aftertreatment, carry on ear good luck to each experiment rabbit the vein take blood and adoptMao allied the immunity adsorb experiment, measure to test rabbit serum in5-the Qiancolor An(5-HT), dopamine(DA), go to AN adrenaline(NE) and β-inside the nerves like FeiMao(β-EP),etc pass the content of quality.Random sentence to death blank matched controland model matched control to test rabbit2for each after building the mold24hs, randomsentence to death each experiment rabbit4for each after curing after3time, take to test themuscular tissue that the rabbit hurts part, Be dyed by HE, just observe the organizationpathology of hurting the part change under the optical microscope.Result:①After tuina cure, tuina treatment set the β-EP content obviously heat up andrelatively have a pole and show Zhao difference with model matched control in the middleof the week blood outside the experiment rabbit;(P<0.01)5-the content of HT, DA and NEobviously lower and relatively have a pole to show Zhao difference with model matched control.(P<0.01).②Organize a pathology research to suggest, after tuina skill cure a3ds,tuiNE treatment set experiment rabbit’s hurting partial muscular tissue basic instaurationhas already been normal, relatively had obvious difference with model matched control.③Tuina town pain function time’s research result of the regulation suggest, the Chinesemanipulative therapy hurts to test to the impatient soft organization rabbit serum in5-themetabolism of HT, DA, NE and β-EP regulate a half-life distinguish are2.71hs,2.0hs,2.04hs and3.16hs.Conclusion:①The passes the research that the Chinese manipulative therapy hurts totest the nerve in the week blood outside the rabbit to pass quality content influence to theimpatient soft organization, and organizes a pathology research and proves that the Chinesemanipulative therapy has a good treatment function to the impatient soft organization harm,at promote muscular tissue instauration of at the same time, can pass to regulate5-thecontent of HT, DA, NE and β-EP but exertion town pain function;②Pass pain function inthe tuina town the research of regulation suggest for time, Chinese manipulative therapyoutward the nerve in the week blood pass the metabolism of quality content to regulate ahalf-life about is a2~3hs, hint tuina clinical treatment the impatient soft organizationhurts of in the process, the skill is proper light and soft to alleviate, cures number of timeswith2times|d is proper, can more availably raise the treatment level that impatient softorganization hurts. |
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