Studies On The Chemical Constituents And Biological Activities Of Abies Fargesii

Abies fargesii belongs to Abies genus of the Pinaceae family, which distributes in the center and west of China. To search for bioactive components, a systematic investigation on its crude extract was carried out. Silica gel column chromatography, thin-layer chromatography, sephadex chromatography, ODS column chromatography, HPLC were used and12compounds were isolated. They were identified by analysing spectra data and comparing with the laboratory samples isolated before. They are24(E)-lanosta-8,24-dien-3,23-dion-26-oic acid (1),23-hydroxy-3-oxolanosta-8,24-dien-26,23-olide (2), maltol (3), vanillin (4),p-hydroxybenzaldehyde (5), p-methylacetophenone (6),p-methoxybenzoic acid (7),(E)-p-methoxycinnamic acid (8),(+)-(3S,4S)-3,4-dihydro-6-methoxy-2H-1-benzopyran-3,4-diol (9), mangifernoic acid (10),β-sitosterol (11), daucosterol (12).Selected compounds were tested for their neuroprotective potential against CoCl2, H2O2, Aβ25-35-induced cell injuries in SH-SY5Y cells respectively. Compound1and3exhibited apparent neuroprotective effects against CoCl2or H2O2-induced SH-SY5Y cell injury. Compound3displayed modest anti-oxidative activity against A β25-35-induced SH-SY5Y cell injury.