Chemical Constituents And Bioactivity Of Abies Chensiensis

Abies is an important genus of the Pinaceae family. There are about 50 species occurring in the highlands of Asia, Europe, North and Middle America, and North Africa. The crude extracts and metabolites have been found to possess various bioactivities including insect juvenile hormone, antitumor, antimicrobial, anti-ulcerogenic, anti-inflammatory, antihypertensive, antitussive, and CNS activities. Abies chensiensis Van Tiegh. (Pinaceae) occurs exclusively in Qinling region of China. This dissertation reported phytochemical study on this plant and anti-proliferative activities against human tumor cells as well as inhibitory activities against LPS-induced NO production in macrophagesA systematically phytochemical study was carried out, which led to the isolation and identification of seventy-five chemical constituents including triterpenoids, diterpenoids, norditerpenoids, lignans, flavanoids and phenolics etc. Among these compounds, six were new lanostane terpenoids (neoabieslactone A–F, 1–6), two were new biflavonoids (3-epilarixinol and 3,2'-epilarixinol, 25 and 26). In the anti-proliferative test against human tumor cells, the CHCl3-soluble fraction of its crude extract showed potent anti-proliferative activities against four human tumor cells, especially for LOVO (IC50 = 12.5μg/mL). Neoabieslactone F (6) showed the strongest cytotoxicity against Colo205 and MDA-MB-435 tumor cell lines with IC50 values of 7.22 and 9.77μg/ml, respectively. In bioassay against LPS-induced NO production in RAW 264.7 macrophages, the CHCl3-soluble fraction exhibited moderate activity with inhibition rates of 83 % and 12 % at concentrations of 400 and 80μg/mL, and 12-Hydroxydehydroabietic acid (16) show significant anti-inflammatory effect with the IC50 values of 27.7μg/mL.