Protective Effect Of Lingonberry On Neurocyte Damage And Analog Military Stressed Mice

Berries and Pinaceae are rich in bioactive substances, which possess antioxidant,antitumor, and immune enhancement function. This study investigated the antioxidantactivity of the extracts from6kinds of berries and Pinus konaiensis in vitro and itsprotective effects on PC-12(simulation of neural cell) and brain cell from oxidativedamage. Screening the Lingonberry of which have highest neuroprotective effect,then identify its basis. Verify the protective effects of Lingonberry from militarystress by Simulation of military stress model. These studies are of significant meaningin research and develop a safety and effective natural military stress protective agent.We selected the Lingonberry, Lonicera eduils, Sorbus pohuashanesis and other6kinds of berries and Pinus konaiensis as material, using60%ethanol assist withultrasonic to extract, choosing the hydroxyl radical and ABTS radical scavengingcapacity to determine the antioxidant activity in vitro. Results suggest that theantioxidant capacity of Lingonberry, Lonicera eduils, blueberry, Sorbuspohuashanesis is higher than others. Using MTT assay to evaluate the effect of7extracts on the survival rate of PC-12and brain cell damaged by hydrogen peroxide,results show that Lingonberry, siberian crab, Pinus konaiensis extract is better inimproving the survival rate of PC-12and brain cell damaged by hydrogen peroxide.According to results of the antioxidant activity in vitro of those7extracts andtheir protective effect on PC-12and brain cell by oxidative damage, we concludedthat Lingonberry have the best neuroprotective effect. And using HPLC quantitativeanalysis analyze the Lingonberry extracts enriched by X-5macroporous resin, weobtained4main components, they are chlorogenic acid, delphinidin, cyaniding andpelargonidin, and their content are52.1898μg/mg,22.4422μg/mg,0.6989μg/mg and6.2933μg/mg, respectively，The ratio of these ingredient content is75.8:32.1:1:0.1。We also established a Simulation of military stress model in this study and usingthis model to evaluate the protective effect of Lingonberry on military stress. Aftercontinuous administration of Lingonberry extract on mice, we found that Lingonberryextract have a different degree protective effect on the mice immune organs such asliver and spleen. And it improves the SOD and GSH-Px activity of the mouse braintissue, reduces the content of lipid peroxidation MDA. The results show that Lingonberry could inhibit the oxidative damage in brain tissues by enhancing theactivity of antioxidant enzyme. Besides, Lingonberry extract can also significantlyinhibit the increase of glucocorticoid in model mouse, increase the content ofmonoamine neurotransmitter5-HT and decrease the content of lactic andacetylcholinesterase in some extent. Therefore, we draw the conclusion thatLingonberry have an effect on the regulation of relevant hormone in organism, andeven have relation with free radical scavenging in the nervous system. Through theresearch on the protective effect of Lingonberry on military stress model, we verifiedthat Lingonberry could reduce and alleviate the adverse effect with uncomfortablemood, cognition, fatigue induced by military stress.