Protective Effect Of Indomethacin In Renal Ischaemia-reperfusion Injury In Mice

Objective:To evaluate the renoprotection effects of non-steroidal anti-inflammatory drugs (NSAIDs) in renal ischemia-reperfusion injury (IRI), and the COX1/2blockade association by indomethacin (IMT) in mice model.Methods:IMT was administrated after the left renal pedicle was clamped with four doses of IMT (1,3,5and7mg/kg) by intraperitoneal injection. Blood and kidney samples were collected24hours after IRI. The renal functions and the cytokines were assayed creatinine in the serum by using ELISA kits. Kidney samples were analyzed by hematoxylin and eosin (HE) and immunohistochemistry staining.Results:The mice administered with IMT (5mg/kg) had a marked reduction in serum creatinine and significantly less tubular damage. The tumor necrosis factor a (TNF-a) activity in renal homogenates and interleukin6(IL-6) activity in serum had a marked reduction at doses of IMT (5,7mg/kg). The administration of dosages (3,5mg/kg) had a marked reduction in the ratio of Thromboxane B2to6-keto Prostaglandin F1α. COX1and2staining was weaker in IMT (5mg/kg) groups than in the other groups.Conclusion:There was a dose-response in the IMT function of renal ischemia reperfusion injury in mice, and IMT had a protective effect in acertain dose range. The effects of IMT on mice IRI was related to COX1and2blockades.