The Mechanism Of Protective Effect Of Proanthocyanidins In Mice Against Chemotherapy Injury

ObjectiveThe project aims to establish a mouse model of chemotherapy damage, examine whetherproanthocyanidins can reduce chemotherapy-induced bone marrow suppression, liver damage,immune suppression and other side effects, and to explore possible mechanisms to provideexperimental and theoretical basis for further development and application of medicinal value.Methods and Result1.Protective effect of procyanidins on mouse bone marrow suppression with chemotherapy.The96ICR mice were by randomized into a blank group,a model group, Positivecontrol(Zhen qi fu zheng jiao nang),and a Proanthocyanidins (high,medium and low dose) group.“Zhen qi fu zheng jiao nang” group and Proanthocyanidins groups mice according to thecorresponding dose feeding every other day for three weeks, the control group and model groupwere received an equal volume of saline, Three weeks later, except for the blank control groupwere given intraperitoneal cyclophosphamide100mg kg-1for three day, to induce an animalmodel of chemotherapy injury,the mice in control groups were gavage with0.9%saline, The“Zhen qi fu zheng jiao nang” group and Proanthocyanidins group mice continues to beadministered according to the original dose. It last1、7days, respectively, After administration ofdrugs，we detected peripheral hemogram, body weight, bone marrow nucleated cell, thymusindex,spleen index and histopathological changes of Bone marrow tissue. The results showed thatmedium-dose and high-dose proanthocyanidins can effectively improve cyclophosphamide in miceinduced bone marrow suppression, compared with the model group, the mice were fedproanthocyanidins lighter weight loss, peripheral blood cells, thymus index, bone marrownucleated cells were has increased（P＜0.05）, and bone marrow tissue damage lighter.2. Protective effect of proanthocyanidins on mouse liver injury and immunosuppressive withchemotherapy.The48ICR mice were randomly divided into six groups, the control group, model group,DDB Group, proanthocyanidins groups (high, medium and low dose). GPC group and DDB groupby feeding each dose every other day for3weeks, the control group and model group were givennormal saline.Three weeks later, except for the blank control group were given intraperitoneal cyclophosphamide60mg kg-1on1、3、5、7day, The Proanthocyanidins group mice and theDDB group mice continues to be administered according to the original dose,2hours after the lastadministration to detect mouse thymus index, spleen index, serum ALT levels, MDA content inliver tissue, liver tissue SOD activity, and histopathological observations of liver and spleen.Results,compared with the model group, proanthocyanidins can significantly reduce serum ALT,liver tissue MDA content, SOD activity increased in liver tissue, improve thymus index（P＜0.05）and reducing loss of spleen lymphocytes,significantly improved liver damage and immunesuppression caused by cyclophosphamide.Conclusion1. proanthocyanidins mitigate myelosuppression caused by cyclophosphamide in mice byincreasing the thymusindex，spleen index, bone marrow nucleated cell count, white blood cellcount and reduce the damage of bone marrow cells.2. Proanthocyanidins on cyclophosphamide-induced liver injury has a significant protectiveeffect, the mechanism may be enhanced antioxidant proanthocyanidins liver, reduce oxidativestress damage.3. Proanthocyanidins improve immunosuppression caused by cyclophosphamide issuppressed by improving immune mouse thymus index, spleen index, reducing spleenlymphocytes missing.