Experimental Research On Secondary Brain Injury And Protective Mechanism After Intracerebral Hemorrhage

ǔObjectiveǖ: By observing the dynamic expression of OX42, nuclear factor-kappaBp65(NF-κBp65), brain-derived neurotrophic factor(BDNF), synaptophysin(SYP),Caspase3following ICH in rats and the intervention effect of An Nao Wan(ANW) on theirexpression, to explore the protective mechanism of ANW on secondary brain injury afterICH.ǔ Methods ǖ:190SD male rats were randomly separated into normal group,sham-operation group(sham group), ICH model group(model group) and ANW interventiongroup(ANW group), the last three groups were randomly divided into6subgroupsrespectively at12h,1d,2d,4d,7d,10d after the establishment of animal model. The modelsof ICH were established by Rosenberg methods. ANW was given to the ANWgroup(1mg/ml,10ml/kg) twice a day. The neurological deficit scores of rats were observedon each group respectively; the expression of OX42, NF-κBp65, BDNF, SYN, Caspase3were detected by immunohistochemical methods and the expression of BDNF, Caspase3were evaluated by Western blot method.ǔResultsǖ: The neurologic deficit scores of rats in ANW group were lower than Modelgroup(P<0.05). The results of immunohistochemical method showed that: little expressionof OX42-positive microglia appeared in normol group and sham group, lots of OX42-positive microglia appeared in perihematoma region at12h after ICH, peaked at7-10d, the expression and activation of OX42-positive microglia in ANW group weresignificantly lower than model group at different time points(P<0.01); the expression ofNF-κBp65in model group increased at12h after ICH, peaked at4d, the expression ofNF-κBp65in ANW group were significantly lower than model group at each timepoint(P<0.05); the expression of BDNF in model group increased at12h after ICH, peakedat1d, the expression of BDNF in ANW group were significantly higher than model groupat each time point(P<0.01); the expression of SYN protein in ANW group were higher thannormal group at1d,2d,4d,7d,10d after ICH, and there was a statistically significantdifference(P<0.01); the expression of Caspase3in model group and ANW group increasedat12h after ICH, the expression of Caspase3in ANW group were significantly lower thanmodel group at each time point(P<0.01). The Western blot showed that the expression o fBDNF protein in model group obviously increased at12h after ICH, peaked at1d, theexpression of BDNF protein in ANW group were significantly higher than modelgroup(P<0.01); the expression of Caspase3protein in model group increased at12h afterICH, peaked at1d, the expression of Caspase3in ANW group were obviously lower thanmodel group at different time points(P<0.01).ǔConclusionsǖ: After ICH microglia was activated and NF-κBp65, BDNF, SYN,Caspase3were highly expressed. ANW has protective effect on brain after ICH, the effectmay work by inhibiting the activation of OX42, decreasing the high expression ofNF-κBp65and Caspase3protein, increasing the expression of BDNF and SYN protein.