The Experimental Influences Of Baihui-Qubin Acupuncture On Brain-Derived Neurotrophic Factor Expression Of Rats With Intracerebral Hemorrhage

Objective:To research the mechanism of cerebral protection of acupuncture and to direct the clinic treatment,through the experimental study of BaiHui-QuBin scalp-acupuncture on immunohistochemical method of Brain-derived neurotrophic factor after intracerebral hemorrhage in rat.Method:1.Totally 60 male wistar rats were randomly allocated into four group:blank group(n=6),intracerebral hemorrhage control group(n=18), acupuncture group(n=18),and western medicine(pisacetam) group(n=18).2.The rat models of acute intracerebral hemorrhage were made.3.The acupuncture point:BaiHui point to QuBin point,which can penetrate three regions of the cupula and forehead and temple.4.The Berderson neural deficit scores were tested at three time points(6h,2d,7d after hemorrhage model establishment) before the rat models were killed,then the brains were removed and index were detected.5.The average gray scale of BDNF by immunohistochemical method were checked,the morphologic change on light microscope was observed,.6.Statistical treatment was made.Results:1.The detecting result of BDNF with the methods of immunity histochemistry:In the model set and the pinprick set,the expression of BDNF of rat's brain tissue surrounding the hematoma was increased gradually and to the peak in 2 days with the increasing of different time phase point,but decreased gradually in 3 days and 7 days.In the same time phase point,Compare with the model set,the pinprick set's expression of BDNF was higher obviously.Both were relatively showed the significant difference(p＜0.01) and have the statistical significant.In the same time phase point,Compare with the western medicine set,the pinprick set's expression of EAA was lower obviously.Both were relatively showed the significant difference(p＜0.01) and have the statistical significant.2.The results of the neurologic deficit score of the acupuncture group is better than intracerebral hemorrhage group and western medicine group at 2 days.Acupuncture group and western medicine group were better than those in intracerebral hemorrhage group at 7 days.3.The results of the brain pathological change in acupuncture group and western medicine group is different from those in intracerebral hemorrhage group.Conclusion:1.The acupuncture of BaiHui-QvBin scalp cave on the rat of the experiment intercerebral hemorrhage can promote the expression of BDNF of rat's brain tissue surrounding the hematoma.2.Acupuncture scalp cave on the rat of the experiment intercerebral hemorrhage can relieve the extent of the neurologic impairment.