Studies On The Alcoholic Sobering Effects From Hovenia Acerba Lindl

Hovenia is considered as one of the widespread use traditional herbal medicines overthe past several decades, especially indigenous in China, Japan, Korea and the Himalayaareas. Although the most of the studies on this species are in Asian countries, it isconcluded that this species contains numerous types of active compounds, the main ofwhich include alkaloids, flavonoids, and triterpenoid saponins. Hovenia acerba Lindl. is thedried ripe seed of a member of family Hovenia. It’s reported by domestic and internationalclinical pharmacology experiments that the active components extracted from Hoveniaacerba Lindl. can be consumed as a hepatoprotective drug of protecting liver after alcoholicpoisoning due to their hepatoprotective activity and the redundancy of alcoholconcentration in the blood. Additionally, other bioactivities of crude Hovenia extractionshave been investigated by the modern studies based on various techniques, includingalcohol detoxification effect, sweet-taste inhibiting effect, anti-allergic activity,anti-microbial effect, anticancer effect, anti-lipoperoxidation effect, and anti-diabetic effect.This article summarizes three parts of Hovenia species, including the main chemicalcompounds, quality control and dihydromyricetin. Based on the sysmatically review,experiments are conducted to invest the isolation and elucidation of chemical constituentsfrom Hovenia, the quality control of dihydromyricetin from Hovenia, chemical conversionmethod of dihydromyricetin from Hovenia and Hovenia’s alcoholic sobering effects.Firstly, the extraction process trial of dihydromyricetin from Hovenia discussed threeInfluencing single factors: methanol dosage, extraction times and extraction period.Method: to determine the optimum process conditions by designed orthogonal experimentbased on the the several single factors. Result: the optimum extractional conditions of dihydromyricetin from Hovenia are:10times methanol, three times extractions,2hourseach time, which are considered to be precise accurate, recurrence and stability.Secondly, in order to improve the yield of dihydromyricetin from Hovenia, myricetinwas transformed into dihydromyricetin by using reducing agents: nickel chloride/sodiumborohydride and bromine chloride/sodium borohydride, with discussion of the influenceof time, tempreture and ratio of reducing agents. However, due to the experimentaldifficulty, hard-controlled condition and no refenrence, the experimental results did notachieve the desired effect. It was possible but not definite that the choosed reducing agentswere not suitable reductants for the transformation from myricetin into dihydromyricetin.Thirdly, as the result of a systematical investigation of the chemical constituents fromthe seeds of Hovenia acerba Lindl，3compounds were isolated by using open-colum (SiO2)which were identified as Hovenitins Ⅰ (Ⅰ), Laricetrin(Ⅱ), Dihydromyricetin (Ⅲ).Furthermore, the mice’s condition of drunk and sober was determined by the overturnexperiments. Gavage dose of0.32ml/20gig50%liquor was given to experimental mice thatcan be denified as the drunk dose because mice’s overturn reflex disappered, showing adrunken state, and no mice died. The result showed that ethyl acetate extract from Hoveniahad significant alcoholic sobering effect, and before drink.