MDM2，P53,Ki-67,Protein Expression In Oral Squamous Cell Carcinoma And Its Significance Summary

Objective:Immunohistochemical method was used to detect oral squamous cell carcinomapatients and observe MDM2, P53, Ki-67protein expression in healthy patients with oralsquamous cell carcinoma patients to explore the MDM2, P53, Ki-67protein expression inoral squamous cell carcinoma and clinical significance for the diagnosis.Method:By immunohistochemistry MDM2，P53and Ki-67protein in20cases of normalmucosa, oral leukoplakia and oral squamous cell carcinoma, stain and positive cell countswere observed between the positive expression rate of each group, at the same timecalculation, using SPSS17.0statistical software to analyze the data statistically amonggroups.Results:(1) The positive rate of MDM2in normal mucosa, oral leukoplakia and oral squamouscell carcinoma were0%,55.00%, and90.00%. The difference between each group wasstatistically significant (P <0.05). The positive expression rate of P53in normal mucosa,oral leukoplakia and oral squamous cell carcinoma were5.00%,30.00%, and70.00%,which was also statistically significant between groups (P <0.05). Ki-67expression innormal mucosa strength in low, medium and high proportion is95.00%,5.00%,0%, Ki-67in mucosal leukoplakia expression intensity in low, medium and high order proportionis50.00%,25.00%,25.00%, Ki-67expression in oral squamous cell carcinoma of theintensity in low, medium and high proportion is15%,15.00%,70.00%and difference ofpositive expression rate of each group was statistically significant (P <0.05).(2)MDM2and P53correlation analysis: the expression intensity of MDM2and P53increased in theoral squamous cell carcinoma along with the clinical pathology graduation increased. Theirexpression was positively correlated (r=0.509, P=0.022). In addition, a positive correlation of MDM2and P53expression was also observed in the oral leukoplakia (r=0.667,P=0.001);(3) MDM2and Ki-67correlation analysis: MDM2and Ki-67expressionincreased in the oral squamous cell carcinoma along with the clinical pathology graduationincreased. Their expression presented a positive correlation (r=0.793, P<0.01). Similarly, apositive correlation of MDM2and Ki-67expression was also observed in the oralleukoplakia (r=0.704, P=0.001);(4) P53and Ki-67correlation analysis: P53and Ki-67expression strengthened in the oral squamous cell carcinoma along with the clinicalpathology graduation increased. Their expression showed a positive correlation (r=0.728,P<0.01). Similarly, a positive correlation of P53and Ki-67expression was also observed inthe oral leukoplakia (r=0.734, P<0.01).Conclusion:（1）High expression of MDM2, P53and Ki-67protein in oral leukoplakia and oralsquamous cell carcinoma could be used as an indicator of the degree of malignancy of oralsquamous cell carcinoma lesions. MDM2, P53and Ki-67protein abnormal expression maybe related with the development of oral squamous cell carcinoma.（2） Overexpression of MDM2, P53and Ki-67protein may be related with oralleukoplakia dysplasia and oral squamous cell carcinoma clinical pathological grade.（3）MDM2expression was positively correlated with P53and Ki-67.