| `This study was to investigate Poplar bark lipid and cell transfer factor on SPFchickens growth performance, immune organ index, cellular and humoral immunefunction effects.Test1Test a poplar bark lipid extraction and cell transfer factorPeel fresh poplar broken, crushed dried, extracted with petroleum ether, filtered,and then dry the petroleum ether to obtain a more concentrated flavor tree wax darkbrown oily liquid lipid poplar bark; then subjected to composition analysis. We canobserve the following results: Vitamin E1590.00mg/Kg, β-carotene60.00mg/Kg,lecithin1.10%,1.09%, sterols, fatty acids,71.3%,1.18%polysaccharides,glycosides1.1%, crude protein2.5%. Thawing frozen chicken spleen taken, cuttinginto pieces and homogenizing after addition of water, six times repeated freezing andthawing, centrifugation, ultra-filtrate obtained after ultra filtration is transfer factor,and then component analysis results come as follows: ribose123.4μg/mL,polypeptide2340μg/mL.Test2poplar bark lipids and cell transfer factor on SPF chickens growthperformanceTest selects of a day-old SPF chicken1500, which were randomly divided intofive treatments with three replicates of100chickens, feeding trials for six weeks.Treatment1was the control group fed the basal diet; handling two basal diet andinjecting Newcastle disease vaccine; handling3in basal diet supplemented with0.3%poplar bark lipids and injecting Newcastle disease vaccine; handling four groups basaldiet, in the drinking water were added to cell transfer factor (1to21days:0.125mL/only;22to42days of age:0.25mL/only) and injecting Newcastle disease vaccine;treating basal diet group5Adding0.3%lipid poplar bark, drinking water was addedto cell transfer factor (1to21days:0.125mL/only;22to42days of age:0.25mL/only) and injection of Newcastle disease vaccine. The results showed that:(1) inpoplar bark dietary lipids can significantly improve the42-day-old SPF chickens bodyweight and daily gain (P<0.05), lower feed conversion, daily feed intake but notsignificantly increased (P>0.05);(2) in the drinking water cytokines increased42-dayweight70g; ADG increased1.68g, average daily feed average intake decreased0.91g,but feed conversion ratio returned to control levels;(3) in feed (0.3%) poplar barklipids, while in the drinking water of cytokines (1to21days:0.125mL/only;22to42days of age:0.25mL/only), so42days age body weight and daily gain increased significantly (P<0.05), daily feed intake and feed conversion did not changesignificantly (P>0.05). The experiment showed that adding a separate lipid and poplarbark poplar bark lipids and cell transfer factor while adding, you can make to improvethe growth performance of SPF chickens; adding cytokines alone does not reduce thegrowth performance of SPF chickens.Test3poplar bark lipids and cell transfer factor on SPF chickens immune organIndex.Test2a breeding experiment in the first14、28、35、42days were randomlyselected from12(per replicate1) close to the average body weight of chickenssacrificed, thymus, spleen and bursa, measured thymus, spleen and bursa immuneorgan index. The results showed that:(1) in the basal diet supplementing with0.3%poplar bark lipids, in addition to42days of age compared with the control group,spleen index increased significantly (P<0.05), the entire growth cycle of immuneorgan index was not significant (P>0.05), but are slightly elevated trend;(2) addingcytokines drinking water (1to21days:0.125mL/only;22to42days of age:0.25mL/only) after28days, compared with the control group compared to the bursal indexsignificantly increased (P<0.05);42days of age, spleen and thymus index increasedsignificantly (P<0.05); entire growth cycle immune organ index was not significant (P>0.05) but both a slight increase trend (3)0.3%in the feed poplar bark lipids, while inthe drinking water of cytokines (1to21days:0.125mL/only;22to42days of age:0.25mL/only),21days, comparing with the control group in the bursa indexsignificantly increased (P<0.05);42days of age, spleen and thymus index increasedsignificantly (P<0.05); remaining growth stage effects on the immune organ index notsignificant ((P>0.05) but slightly increasing trend. In summary, this experimentshowed that poplar bark, either by adding lipids, cell transfer factor, or bothsimultaneously added, SPF chickens’ immune organ index has improved.Test4poplar bark lipids and cell transfer factor on cellular immune effect SPFchickensFeeding trials were tested a7days,28days,42days each repeat randomly taken10wings vein blood, EDTA anticoagulant blood T cell subsets was measured (CD4+,CD8+) the number and percentage of change. The results showed that:(1) in the dietssupplemented with0.3%poplar bark lipids, can significantly (P<0.05) increase SPFchicken peripheral CD4+、CD8+T cell subsets and the number of CD4+/CD8+ratio;(2) in the drinking water cell transfer factor (1to21days:0.125mL/only;22to42 days of age:0.25mL/only), seven days old, on chicken peripheral blood CD4+T cellsSubsets content significantly affected (P<0.05), while the CD8+T and CD4+/CD8+was not significant (P>0.05), but there is a rising trend. At28and42days forchicken peripheral CD4+T, CD8+T lymphocytes and the number of CD4+T/CD8+Tare significantly (P<0.05) improved impact;(3) In feed0.3%poplar bark lipid transferfactor while using cell water (1to21days:0.125mL/only;22to42days of age:0.25mL/only), so SPF chicken peripheral CD4+T cell subsets in the number ofincreased significantly (P<0.05); at42days of age, the SPF chicken peripheral CD8+T lymphocytes was significantly increased the number (P<0.05), on the CD4+/CD8+has significantly increased (P<0.05). The experiment showed that adding either alonepoplar bark lipids, cell transfer factor, or both simultaneously added, can improve theSPF chicken peripheral CD4+、 CD8+T cell subsets and the number of CD4+/CD8+ratio.Test5poplar bark lipids and cell transfer factor on SPF chickens humoraleffectsFeeding trials were tested a7days,28days,42days each repeating randomlytaken10wings vein blood serum was separated, the detection antibody titers; collecttears and intestinal SPF chickens were determined IgG, IgM, IgA content resultsshowed that:(1) in diets supplemented with0.3%poplar bark lipids, significantlyincreased antibody levels (P<0.05),7days old, SPF chickens tears of IgAsignificantly increased (P<0.05),28days old, SPF Intestinal fluid IgG, IgMsignificantly increased (P<0.05);(2) in the drinking water cell transfer factor (1to21days:0.125mL/only;22to42days of age:0.25mL/only), SPF chickens tears of IgG,IgM, IgA significantly increased (P <0.05), intestinal fluid IgG significantly increased(P<0.05),28days old, the antibody levels were significantly increased (P<0.05),;(3)0.3%in the feed while using cell poplar bark lipid transfer factor drinking water (1to21days:0.125mL/only;22to42days of age:0.25mL/only), antibody levels weresignificantly increased (P<0.05),7days and28days of age, on the SPF chickenstears in IgG improved significantly. The experiment showed that adding either poplarbark lipids alone, cell transfer factor, or both adding in SPF chickens can slightlypromote the role of humoral immunity. |
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