| This thesis includes the introduction, the composite film prepared using urushioland Ru (bipy32+doped silica nanoparticles as a novel adhesive for modified electrode,and the researches of electrogenerated chemiluminescence analysis （ECL） behavior ofthe modified electrode with the composite film using urushiol and Ru (bipy32+dopedsilica nanoparticles as a novel adhesive, the researches of flow injection-electrogenerated chemiluminescence analysis behavior.Chapter1is a summarization, which includes the introduction of ECL, theprinciple of flow injection method, the developing status, current status and theapplication of chemically modified electrode, the modified electrodes with Ru （bipy）32+and its application in pharmaceutical analysis, and research progress of Chineselacquer.Chapter2, the researches of ECL behavior of the modified electrode with thecomposite film using urushiol and Ru(bipy32+doped silica nanoparticles as a noveladhesive.The modified electrode were prepared using the composite film of urushioland Ru(bipy32+doped silica nanoparticles as a novel adhesive. Moreover, thedifferences in the analysis of the modified electrode and the bare glassy carbonelectrode were compared in order to its effective application in the electrochemistryanalysis.Chapter3, the ECL behavior of the chemiluminescence sensor prepared usingmodified electrode with the composite film of urushiol and Ru(bipy32+doped silicananoparticles as a novel adhesive with coreaction reagion of tripropylamine andascorbic acid, the limit of detections of tripropylamine and ascorbic acid are2.1×10-7mol/L and2.5×10-8g/mL, respectively. The sensitivity is higer than that ofliterature reports. It shows that with the method of new type of modifed electrodematerials studied in the experiment, Ru (bipy32+can be fixed on the electrode surface.In this way, we can prepare an effective and good charge-transfer function of sensor,which can be used to determination of drug samples.Chapter4, the determination of trace magnolol using the chemiluminescencesensor prepared using modified electrode with the composite film of urushiol and Ru(bipy32+doped silica nanoparticles as a novel adhesive. The each parameters ofchemiluminescence system were optimized. Under optimal conditions, the signalstrength of the system reached the maximum in order to improve the sensitivity. ECLintensity is proportional to the concentration of magnolol in the range of 8×10-78×10-5mg/mL. The linear regression equation is I=1.97×106c（mg/mL）+96.91,correlation coefficient r=0.9915, the limit of detection is3.7×10-8mg/mL, and therelative standard deviation （RSD） of eight determinations of magnolol atconcentration of8×10-6mg/ml was3.5%.Chapter5, the determination of vanillin with FIA-CL method. Under acidicconditions, vanillin have significantly weakened on the chemiluminescence ofRu(phen32+-Ce（Ⅳ） reaction system, whereby a new system determination ofvanillin with the flow injection-chemiluminescence was developed. Various factorsthat affect the flow injection chemiluminescence experimental were studied tooptimize the reaction conditions and the determination of the parameters. The resultsshowed that under the optimized condition, in the mass concentration range of4.00×10-78.25×10-6g/mL, the relative luminous intensity and concentration was alinear relationship in logarithm, the detection limit （3σ） was2.90×10-7g/mL. The RSDof11determinations of Vanillin at mass concentration of3.0×10-6g/mL was2.6%, andthe recoveries of determination of vanillin in synthetic samples were between98.3%and102.8%under sampling frequency of120times/h. |
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