The Study Of Solid Electrochemiluminescence Sensor Based On Carbon Nanofiber

The immobilization of tris(2,2'-bipyridyl) ruthenium(II) (Ru(bpy)32+) on an electrode surface can reduce the consumption of reagent, simplify the experimental design, and facilitate miniaturization. In this thesis, we developed a novel solid-state electrochemiluminescence (ECL) sensor based on the carbon nanofiber (CNF) for the determination of atropine in real sample. This proposed sensor was also applied for capillary electrophoresis-ECL system (CE-ECL). The main work of this thesis is summarized as follows:1. Ru(bpy)32+ was immobilized in Nafion/CNF composite films by the electro-static absorption, which was then modified on the carbon paste electrode to fabricate the ECL sensor. Then the proposed sensor was used to detect atropine. We studied voltammetric and ECL behaviors of immobilized Ru(bpy)32+, and investigated the effects of the CNF amount in the composite films, the volume of the CNF/Nafion composite solution dropped on the electrode, immobilization time and solution pH on the detection of atropine. Under the optimized conditions, we obtained low limit of detection (LOD), wide linear range and good repeatability. The ECL sensor was also demonstrated for the determination of atropine in human urine sample and satisfactory results were obtained.2. The ECL sensor was successfully used in the CE-ECL system to analyze atropine. The CNF/Nafion/Ru(bpy)32+/CPE was used as the working electrode, which could effectively reduce the consumption of expensive reagent.