DNA Sensors Based On Electrochemiluminescence For The Detection Of TATA Binding Protein

Electrochemiluminescence (ECL) nucleic acid sensor was a kind of high sensitive and high selective detection device, which was widely used in genetic detection, medicinal research, environment monitoring and food security. The interaction between DNA and protein plays a important role in DNA replication, translation, transcription.in this paper Ru(phen)32+and Luminol was chosen as the ECL signal,which was widely used in ECL. We constructed an ECL DNA sensor which has special response for TATA Binding protein (TBP), and used to detect the concentration of TBP.1. Herein, Ru(phen)32+was used to intercalate into double strand DNA (ds-DNA) as an ECL probe with TBP as the target. After the thiolated DNA probes (25-mer) was hybridized with their complementary targets (22-mer). Ru(phen)32+, as the probe, was intercalated into the ds-DNA structure in solution.Then,then ds-DNA was attached to a gold electrode, After TBP bound with its ds-DNA, blocked the electron transfer on eletrode. The difference in ECL intensity with tripropyl-amine (TPrA) as coreactant before and after the binding of TBP and ds-DNA was used to quantify TBP. The method had low detection limits, with0.01nM TBP being detected and also simple experimental procedures using the intercalation of Ru(phen)32into ds-DNA, with no chemical labeling to DNA.2. A novel ECL DNA sensor using Au nanoparticles as signal transduction probes was described for the detection of TBP. The5nm gold nanoparticles were specifically conjugated to the thiol after DNA polymerization process in the presence of Sp-dATP-α-S and Klenow fragment.when the ds-DNA was bound with TBP polymerization can’t occurred. Due to its good conductivity, large surface area and excellent electroactivity to Luminol oxidization, the gold nanoparticles were expected to amplify the ECL signal of Luminol as reported. The device can detected0.5to100nM TBP,which detection limit was higher than the preceding chapter.In this issue,the amplification of ECL signal of Luminol by Au nanoparticles was unconspicuous.then we list the possible reason of potential, Further research needs to be continued in future.