Fabrication Of Signal On/Off-based Electrochemical Or Electrochemiluminescence Sensor For The Detection Of Pesticides

Our country is currently the one of earliest use of pesticides to control crop disease, insects and grass. China is also a big country on producing and using of pesticides. Not only the pesticide residue makes a strong threat to human's life, but also to the export of the fruit and vegetables results. Therefore, rapid determination and reliable quantification of trace level of pesticide residue have become very important for homeland security and health protection. Traditional analysis of pesticide residues is routinely carried out by gas/liquid chromatography or mass spectroscopy. These methods often require complicated pre-treatment steps, extensive labor resources, and are not applicable for on-site determination. Although the in-situ detection of pesticide residues can be achieved for the spectrometry method, the sensitivity is low. To develop a rapid and effective method for detecting pesticide residues is extremely important.Based on the above knowledge, this article focuses on the fabrication of novel Signal on/off-based Electrochemical or Electrochemicluminence sensor for the detection of the pesticides:1. We successfully fabricated the layered double hydroxides (LDHs) decorated Graphene nanosheets (GNs) nanocomposite by a facile co-electrodeposition approach, using exfoliated GO as precursor. Such a nanostructured composite film, combining the advantages of LDHs together with GNs (the efficient capture of OPs and the facilitated electro-transfer processes), leads to a remarkably improved sensitivity and selectivity. The resulting sensor showed both good reproducibility and ideal stability.2. A highly sensitive and selective biosensor has been developed for the determination of methyl parathion, where the smart integration of electrochemically reduced graphene (ERG), QDs and biomolecules acetylcholin esterase (AChE) yields a biofunctional AChE-ERGO-CdTe QDs hybrid as cathodic ECL emitters for OPs sensing. Based on the consumption of dissolved O2 in enzymatic reaction, a highly sensitive signal-on ECL biosensor has been constructed. The resulting biosensor showed good sensitivity, reproducibility, ideal stability and fine applicability for the detection of methyl parathion in real samples.3. A highly sensitive and selective biosensor has been developed for the determination of pentachlorophenol. Based on Molecularly imprinted-luminol anode signal-off ECL biosensor. And the ECL intensity under certain experimental conditions can be effectively inhibited pentachlorophenol (PCP). For this reason we have developed a novel method of analysis to detect PCP. Since luminol electrode MIP/CC can emit light on a strong light intensity, the method can have a detection sensitivity with respect to the PCP of other methods have been reported.