Electrochemiluminescence Immunosensor Based On Gold Nanoparticles Modified Electrode

Electrochemiluminescence immunoassay (ECLIA) is a quantitative measurement method for antigen or antibody that based on electrochemiluminescence (ECL) signal change before and after immunoreactions. ECL immunosensor is a new-style immnuosensor that combine immobilized antigen or antibody on surface of electrode with ECL intensity as detection signal. It has gained considerable interest as the bioanalytical device in recent years, and has been the attractive tool because it has high selectivity, can achieve excellent detection limits with small volumes of analyte and reduce assay time, and is convenient to use.Gold nanoparticles have obtained increasing interest in analytical chemistry field. Gold nanoparticles are characterized by several advantages such as the following: good biocompatibility, conductivity, high catalyze efficiency. It has reported to the applications of gold nanoparticles in electrochemical immunosensor, fluorescence immunosensor, surface plasmon resonance (SPR) immunosensor and piezoelectrical (PZ) immunosensor. However, little work has been done in the application of gold nanoparticles to ECL immunosensor. The aim of the present work is to study enhancement of ECL detection signal at gold nanoparticles modified electrode, and design a high sensitivity, fast, simple ECL immunosensor that based on the specific interaction between antibody and antigen using gold nanoparticles modified technology and luminol labeled antibody technology.The major contents in this thesis are described as follows:Chapter 1Review of applications of the nanoparticles in immunosensor. The principle, catalogue, immobilization method of antigen (or antibody) and regeneration of immunosensor were detailed described. The applications of the diversified nanomaterials in immunosensor were summarized.Chapter 2Electrochemiluminescence detection of ferrocenemonocarboxylic acid at gold nanoparticles modified graphite electrode. A highly sensitive ECL method for the determination of ferrocenemonocarboxylic acid (FMC) was developed at gold...