The Effects Of Nutrition Supplement On Glycogen Storage And Some Rectus Femoris Aerobic Metabolism Enzyme Level At Different Time In Rats

Objective:The study observes the effects of nutrition supplement on energy material storage and some rectus femoris aerobic metabolism enzyme level in rats after three weeks incremental exercise. The goal is to probe how the nutrition to promote the different groups with the related data.Method:90SD male rats were random organized into control group (C), exercise group (S), and exercise and nutrition group (T). Then, the group S and T were random organized into 4 groups each other in average, according to the kill time of rats, which ordered after exercise 0 hour, after exercise 1 hour, after exercise 6 hours and after exercise 24 hours. They have no differences of weight among all the groups. Among them, group S and T were taken treadmill exercise for 3 weeks. They trained 6 days per week which had a rest on Sunday, and one time every day in the morning. They trained 40 min at the first week, and then the time was added to 50 min at the second week,60 min at the last week. The speed of running on the first day was 20 m/min, then, it was increased every two days, and reached 38 m/min in the end. Group T was taken nutrition supplement 15 min before the exercise and 15min after. Group S was the same all, but the supplement was water. Besides, group C was taken the equal water as group S. They were all put to death with 2% sedative pentobarbital sodium after 3 weeks, took the blood, liver and rectus femoris of rats to test liver glycogen, muscle glycogen, glucose, INS, SDH and MDH, and used SPSS13.0 to dispose statistics.Results:(1)Univariated general linear model analysis showed that the main effect nutrition supplement on the liver and muscle Glycogen storage and the level of blood Glucose, serum Insulin, rectus femoris Succinate Dehydrogenas and Malate Dehydrogenase was significant difference between the two groups (P<0.01). (2) One-Way ANOVA showed that the Muscle Glycogen at 6 and 24 hour after exercise was significantly higher than it at the next time within group S and T (P<0.01). The Liver Glycogen at 6 and 24 hour after exercise was significantly higher than it at the next time within group S and T (P<0.01). The Blood Glucose at 1 hour after exercise was significantly higher than it after exercise instantly of group S and T (P<0.01), the Blood Glucose at 6 and 24 hour after exercise was significantly higher than it at 1 hour after exercise of group S and T. The Insulin at 24 hour after exercise was significantly higher than it after exercise instantly and at 1 hour and 6 hour after exercise of group S and T (P<0.01). The rectus femoris Succinate Dehydrogenas at 6 hour after exercise was significantly higher than it after exercise instantly of group S and T. The rectus femoris Malate Dehydrogenase at 6 hour after exercise was significantly higher than it after exercise instantly of group T (P<0.05). (3) T test indicated that the level of Liver Glycogen of group T was significantly higher than that of group S at 6 and 24 hour after exercise (P<0.05). The level of Liver Glycogen of group T was significantly higher than that of group S, after exercise instantly and at 6 hour after exercise (P<0.05). The level of Blood Glucose of group T was significantly higher than that of group S at 1 and 6 hour after exercise (P<0.05). The level of Insulin of group T was significantly higher than that of group S at 1 and 24 hour after exercise (P<0.05). The level of rectus femoris Succinate Dehydrogenas and Malate Dehydrogenase of group T was significantly higher than that of group S after exercise instantly (P<0.05).Conclusion:(1) Taking nutrition supplements of the rats can significantly enhance the capacity of the liver and muscle glycogen recovery, can improve the serum level of insulin secretion after exercise, and can slower decline the level of blood glucose to maintain it density. (2) Taking nutrition supplements of the rats can significantly enhance the capacity of the liver and muscle glycogen and blood glucose recovery at 6 hour after exercise. (3) Taking nutrition supplements of the rats can significantly disease the level of rectus femoris Succinate Dehydrogenas in cytoplasm and increase the level of rectus femoris Malate Dehydrogenase to enhance the energy supply after exercise instantly.