| Sea point is one of the five Shu points. It can treated the main symptom of the disease with counter gas and diarrhea. Low sea point is one of the specific acupoints of human body. Six internal organs related diseases can be treated by low sea points. What are the difference effects between Sea point and Low sea point? what is the difference between their mechanism of action?According to our previous study found, the pathological changes in COPD rat mesenteric microcirculation can be treated better by Chize point than Shangjuxu point. Therefore, we propose a doubt, for all intestinal organs pathological change, the efficacy of Chize the better than Shangjuxu points? This topic is established on the basis of the literature review. Therefore, the present study, experimental ulcerative colitis model to observe the different characteristics of the " Sea points Chize" and "Low sea point Shangjuxu" effect, to discuss the difference of the mechanism of action, and to further explore the relative specificity of acupoints.This paper is mainly divided into two parts of the literature and experimental research.The literatureFirstly an overview of the basic theory of combined points, the combined points described in the classical literature, we found that the combined points, the combined points are vomiting, diarrhea and other diseases can be treated; Chize and Shangjuxu points cases, respectively, of the two points Indications role in animal experiments and clinical application and found that both can be used in the clinical treatment of diseases such as diarrhea, but what is the difference between the two, yet reported in the literature.Thus, we have made a question whether the Sea points and Low Sea points have the same role in the treatment of diarrhea? To get the answer, in this experiment by constructing a rat model of ulcerative colitis, compared electro-acupuncture stimulation at Chize and Shangjuxu rat mesenteric microcirculation changes and multiple organ VIP, TNF-alpha, NPY and colon pathology, and to explore the two points are attending law.Therefore, in the literature, we respectively study the efficacy of acupuncture in the treatment of UC and acupuncture methods, the relationship between the UC rats modeling method as well as an important indicator of the experiment VIP and VIP mesenteric microcirculation.The experimental studyObjective: The observed EA Chize points and Shangjuxu point at the ulcerative colitis rat mesenteric microcirculation, vasoactive intestinal peptide (VIP), Tumor Necrosis Factor (TNF-a), neuropeptide Y (NPY)distribution and rat colon pathological damage in the lung, colon, hypothalamus, the combined points Chize and under the combined points on the giant virtual hole effect differences and mechanisms of the different analysis explore the relative specificity of acupoints lawMethod:28male Wistar rats (body weight to180g±20g)were randomly divided into four groups of normal group, model group, Chize group, Shangjuxu group.Modeling method used for acetic acid enema rat model of ulcerative colitis Chize group, and Shangjuxu group from the3rd day of the experimental period electro-acupuncture acupuncture treatment, density wave, the frequency of2/100Hz alternately, strength muscle trembling slightly as the degree, pulse width0.175ms, left pin15min,1day,7were treated.Observation of rat behavior change, HE staining was observed in colon tissue damage, microcirculation microscopic examination on Measuring rat mesenteric micro vascular diameter, blood flow pattern changes and the lung, colon, hypothalamus organization, with radiation immunosorbent assay in lung, colon, hypothalamus in the VIP, TNF-a, NPY content.Result:1. In general:The normal rats generally in good condition. Model, Chize group and Shangjuxu of group Rats in2days after loose stools, fecal residue around the anus, accompanied by lethargy, anorexia, and so on. Electro-acupuncture treatment for7days, disappear Shangjuxu rats loose stools, loss of appetite and spirit in good condition, Chize rats loose stools disappeared or still soft stools that rats, stool is still forming. Compared with the normal group, three sets of body weight decreased significantly model group, group Chize and the giant virtual group had no significant difference.2. Mesenteric microcirculation:The normal group microcirculation fast, relatively small diameter. Compared with normal group, model group and Chize Group mesenteric microcirculation diameter increased significantly(P<0.05); compared with the model group, the diameter of the Shangjuxu group significantly reduced(P<0.05); compared with Chize group, the diameter of the Shangjuxu group was significantly reduced(P<0.05). Microcirculation flow pattern, see no significant difference in each group. 3. VIP content:(1)between groups:compared with the normal group, the model group Chize group intestinal tissue content of VIP significantly increased(P<0.05); compared with the model group, the giant virtual group intestinal tissue VIP decreased significantly(P<0.05);(2)group comparison:the four groups the hypothalamus contains were significantly higher than the content of the lungs(P<0.05); normal group, group Chize and the giant virtual group, The content of the hypothalamus in the colon content(P<0.05)were significantly higher than,4. TNF-α content:(1)between groups:colon tissue in the model group were significantly higher than normal group, group of Chize and on giant virtual group(P<0.05);(2)comparison group:all groups hypothalamus TNF-alpha were higher than the colon(P<0.05), the hypothalamic within the model group was higher than that of lung tissue(P<0.05); lungs on Shangjuxu group TNF-alpha higher than the colon (P<0.05).5. The NPY content:(1)between groups:no significant difference among the groups.(2)group comparison:In all groups, the content of the lungs was significantly higher than the hypothalamus in the content(P<0.05); colon was significantly higher than in the normal group, the hypothalamus in the content(P<0.05); model group, the lungs were significantly higher than the content of the colon(P<0.05).6. Colon pathological degree of injury:Compared with normal group, model group colon tissue large ulcers in the light microscope biopsy revealed severe colonic mucosal epithelial cell injury. Chize group compared with the model group eased, more complete Shangjuxu group colonic mucosal epithelial cells, no significant damage.Conclusion:Poured into acetic acid through the anus can be successfully replicated the model of ulcerative colitis in rats.2. Shangjuxu points can significantly Inhibitory ulcerative colitis rat colon tissue inflammation, thus more significant pathological changes of the repressor ulcerative colitis rat mesenteric micro vascular diameter.3. Different periods of rats VIP content distribution;6weeks of age, the hypothalamus, the highest VIP content.4. Different modeling methods in inter-organizational variation caused by the same substance. UC rat model group and treatment group, VIP Distribution and Variation between the lung and colon showed the opposite trend, and the COPD rats showed the same trend.5. VIP, TNF-α and NPY in the variation between the lung and colon prove the correctness of the theory Lung and Large Intestine,... |
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