Electric Acupuncture Acupoints On Irritable Bowel Syndrome Model Of Rat Intestinal Microcirculation, Brain Gut Related Research On The Effects Of Neuropeptides

Objective:Irritable Bowel Syndrome(IBS) is a well known functional bowel disorder and a kind of typical Mental and Physical disease. It increased year by year. The pathogenesis is undefined, most of research shows that the pathogenesis of IBS focus on brain-bowel axis disorder and gut feeling high sensitivity. Acupuncture can adjust levels of brain-bowel axis disorder, and acupuncture treatment method is cheap、simple、convenient. So we select Zusanli Point and Tianshu Point to treat IBS model rat by Electric Acupuncture in order to observe the immediate effect, include visceral sensitivity, microcirculation of intestine, bioactive substance in plasma(CGRP、ET、VIP), bioactive substance in colon tissue(NPY、 PGE2、CGRPmRNA、ETR-A). Discuss the part of the mechanism of IBS by acupuncture treatment method and basis of bioactive substance.Methods:Adopt the Vistar newborn rats as IBS model by continuous rectum acetic acid stimulate, separate for4groups:normal group、model group、Zusanli group、Tianshu group. The electroacupunture are given to Zusanli and Tianshu group once from8th week, after treatment, the rats are accept the visceral sensitivity evaluation with the abdominal retraction reflection(AWR), and observed blood flow of mesentery microcirculation in different time period, after that, phlebotomize5ml abdominal aortic blood and take colon tissue. Check CGRP、ET、VIP in rat’s plasma by Enzyme immunoassay method. Check NPY、PGE2、 ETR-A in rat’s colon tissue by Enzyme immunoassay method. Check the calcitonin-gene-related peptide (CGRP) content in rat’s colon tissue by RT-PCR method.Results:1. Estimate on visceral sensitivity of IBS model rats by electroacupuncture:Compared with the normal group, the value of the model group rats’belly raising and back hunch-up significantly reduced (P<0.01). Compared with the model group, the value of the Zusanli group rats’belly raising increased (P<0.05).2. test of the mesentery microcirculation of IBS model rats:①the diameter of the capillaries:compared with the normal group, the diameter of the mesentery capillaries of the model group significantly narrowed in30mins(P<0.01); Compared to the model group, the diameter of the mesentery capillaries of the Zusanli group significantly expanded (P<0.01).②the state of the blood flow:compared with the normal group, the model group’s blood flow speed of mesentery capillaries reduced or stopped (P<0.05).3. Value of CGRP、VIP、ET in plasma:compared with the normal group, The content of CGRP in plasma of model rats were reduce(P<0.05); The content of CGRP in plasma of Zusanli group were ascent (P<0.01), Compared to the model group, the content of CGRP in plasma of Zusanli、Tianshu group was significantly ascend(P<0.05,P<0.01), while for VIP and ET, Compared to the model group, the content of VIP and ET in plasma was significantly descend (P<0.01,P<0.05) in Zusanli、Tianshu group.4. Compared with the normal group, the content of NPY、PGE2in colon tissue of model rats were reduce (P<0.05); Compared to the model group, the content of NPY、PGE2in colon tissue of Zusanli、Tianshu group was significantly ascend(P<0.01).5. Compared with the normal group, the content of CGRPmRNA、ETR-A in colon tissue of model rats were ascent (P<0.05); Compared to the model group, the content of CGRPmRNA、ETR-A in colon tissue of Zusanli group was reduce (P<0.05).Conclusion:1. The stable IBS young rat visceral hypersensitivity model can be built by by continuous rectum acetic acid stimulate.2. Electroacupunture Tianshu、Zusanli point can significantly improve the blood state of the mesentery microcirculation immediately, Zusanli point has stronger effect of pain relief.3. The content of CGRP、VIP、ET、CGRPmRNA、ETR-A、NPY、PGE2disorder, it may be one of mechanism of IBS.4. Zusanli、Tianshu Point leads to difference of acupoint effect, Tianshu point has stronger regulation Of CGRP in the plasma, and Zusanli point has more clearly adjustment action for receptor and gene.