Screening For Asymptomatic Colorectalcancer Above50Years Old And The Relationship Between MiR-21、miR-143and Colorectal Cancer

The incidence rate of colorectal cancer is high,and the disease is harmfulto people.Epidemiological data showed that the incidence of colorectal cancer,mortality rates increased year by year. The occurrence of colorectal cancerdevelopment is a long process, generally speaking,epithelial cells from normalhyperplasia to adenoma and cancer, it takes about10years. In this longprocess, the clinical manifestations of the patients often lack specificity, easilyignored by the patient or family, which caused great pity! Because of diseaseprocesses tardy, providing excellent conditions for clinical intervention.Health service personner through the propaganda of medical knowledge,popularity the awareness of related knowledge, and let the target population ina timely manner.Both to avoid the pain of patients and also reduce health carecosts.At present, our country formulated the guideline of colorectal cancerscreening, prevention and control of a consensus opinion, provide medicalbasis for clinical screening. Opinion shows, combined with the actualsituation in our country, there are two kinds of measures for the screening inlarge population. As the fecal occult blood test (FOBT) and endoscopicscreening. However, the new screening methods are continuously applied toclinical, such as micrnRN-As detection. Invarious provinces and autonomousregions, colorectal cancer screening is following.Objectiv:1This experimental study uses the questionnaire survey and fecal occultblood test,(colloidal gold method)for50years and older with no symptoms inthe general population, screening for early colorectal cancer. As soon as finding high risk crowd and the positive fecal occult blood test person, forelectronic colonoscopy checking, if found lesions, handle in time.2If find adenoma and cancer patients, under the consent of the patienthimself or his family, to return pathological specimens.Using the qRT-PCRtechnology, detect the expression of miR-21, miR-143in normal colon tissue,adenoma tissue and the cancerous tissue, in order to find specific degrees forcolorectal cancer early diagnosis of high biological markers.The experiment includes two parts content.Part one Screening for asymptomatic colorectal cancer above50years old1object In March2012to March2013in the second hospital, hebeimedical university medical center for a medical in the general population.2method After the subjects signed informed consent form andquestionnaire, the right to return a stool specimens, as required to staff. Staffcompleted faecal occult blood test in the prescriptive time. FOBT was told toclient or family member.3result Screening a total of24704people,941people as the immunemethod of FOBT positive, the detection rate of3.87%. Examined forelectronic colonoscopy inspectors, a total of890people,10cases of colorectalcancer, colorectal cancer detection rate of1123/1000,000; Detecting colonpolyps in89cases, detection rate10.0%; Endoscopic treatment of43cases ofcolon polyps, therapy was48.1%; Check out94cases of ulcerative colitis,detection rate of10.6%.4conclusion Asymptomatic healthy physical examination populationaged over50, the immune method of FOBT was accounted for3.81%.Endoscopy in patients with line, check the10cases of colorectal cancer,colorectal cancer detection rate of1123/10; Detect colon polyps in89cases,detection rate10.0%; Endoscopic treatment of43cases of colon polyps, therapy was48.1%; Check out94cases of ulcerative colitis, detection rate of10.6%.Part two The relationship between miR-21、miR-143andcolorectal cancer1object Through the screening, found that positive fecal occult bloodtest client or questionnaire high-risk groups, actively guide the adults forendoscopic examination.2method Under endoscopy, collect10copies of normal colon tissue,10copies of adenoma tissue and10copies of cancerous tissue,then rapidfreezing. Using the qRT-PCR technology for miR-21, miR-143expression.3result In the second part,Detection miR-21in colon cancer andnormal tissue, adenoma tissue, along with the degree of malignancy increased,the expression increase, P <0.05; Detection miR-143in colon cancer andnormal tissue, adenoma tissue, along with the degree of malignancy decreased,the expression decreased, P <0.05.4conclusion MiR21up-regulated in colorectal cancer, miR143down-regulated in colorectal cancer, and may participate in the settlement ofthe occurrence of colorectal cancer development.And, is expected to be as anearly screening for colorectal cancer patients and determine prognosticmarkers.