Effect And Mechanism Of The New Physical Antimicrobial Dressings Prevention Indwelling For Catheter Associated Urinary Tract Infection

Objective:Bacterial biofilm (Biofilm, BF) In vitro experiments and clinical trials, testing new physical antimicrobial excipients (JUC) prevention indwelling catheter the BF formation of the effect to investigate the antimicrobial mechanism.Methods:①In vitro experiments:Establishing catheter BF in vitro model, the experimental group using JUC appropriate treatment, the control group treated with0.9%saline, in a scanning electron microscope (SEM) and confocal laser scanning microscopy (CLSM) was observed under BF morphology.②Clinical trials:expected Urology postoperative indwelling balloon catheter seven days and more than60patients were randomly divided into the experimental group and the control group,30cases in each experimental group catheterization the JUC treatment catheter after with JUC care; care control group with saline conventional urethra, collecting urine prior to catheter removal for bacterial culture were compared the incidence of bacteriuria and observed drug reactions..Results:①In vitro experiments:SEM observation visible catheter surface of7-day control group gathered a large number of bacteria, E. coli culture has formed a mature biofilm of bacteria; catheter surface while the experimental group JUC antibacterial film structure formation, no or only see a small amount of bacterial adhesion. The experimental group latex catheter surface bacterial adhesion, aggregation, initial film-forming to form mature bacterial biofilm was significantly longer than the control group. The JUC is sprayed on the surface of latex catheter, the catheter surface can gradually smooth, is not conducive to bacterial adhesion and colonization; allows E. coli the JUC through physical adsorption deformation, rupture, dissolution, and thus play a bactericidal action. JUC Enterococcus faecalis killing effect. The CLSM observed,7d, the experimental group and the control group compared to the catheter surface polysaccharides and viable cells area, viable cells significantly reduced the proportion of E. coli culture,②clinical trials:the control group urine bacterial culture was positive in eight cases, bacteriuria rate of 26.7%, the pathogens were E. coli4cases, fecal bacteria, Enterobacter cloacae, fungi; experimental group urine bacterial culture positive cases of bacteriuria rate of3.3%, the pathogen E. coli. Bacteriuria incidence of the experimental group was significantly lower than the control group (P<0.05).Conclusion:JUC spray on the catheter surface of physical antimicrobial film can be formed, to be effective in preventing the formation of BF to reduce catheter Associated Urinary Tract Infection (Catheter-Associated Urinary Tract Infection, CAUTI) incidence.