Differential Expssion Of Bcl-xL And Survivin In Colorectal Cancer And Their Expression Correlation Study

Background and Objective: Tumor is formed by the Abnormalproliferation (Neoplastic proliferation) of the body’s cells.Mitochondria wayand death receptor mediated way is the main process of cell apoptosis,eventually start caspase cascade activation and waterfall sample reaction andinduce the apoptosis.Bcl-xL is the most important members of the Bcl-twofamilies,is an isomers which is encoded by Bcl-two gene families and itsstructure and function is similar with Bcl-two,however Survivin (survivalprotein or survival element) is one of the family members of apoptosisinhibiting protein(inhibitors of apoptosis proteins，IAP).Research indicated thatthey all have strong antiapoptotic role.Bcl-xL protein is mainly distributed in the mitochondria outer membrane;it can have stability in the outer membrane mitochondrial function. This can benot only inhibit some apoptosis induced material such as Cyt C released frommitochondria,and still can indirectly inhibit the activation of capspase terminaleffect proteases, and finally paid apoptosis of inhibition.In addition, the studyfound that the Bcl-xL also participate in anti-inflammatory reaction andangiogenesis process.The expression of Survivin highly tissue specificity andtime specificity,only in the human embryonic period organization are widely expression,while in normal adult organization do not express or lowexpression.Survivin may through several different mechanisms directly orindirectly inhibiting apoptosis way caspase3and caspase7terminal effectprotease (cascade enzyme or caspase) activity. Research also found that theSurvivin and favorable to the generation of tumor blood essels, and promote theformation and development of the tumor.Apoptosis is initiative of cells die way, the present study show that theprocess by various gene regulation and control imbalances caused by abnormalprocess is the cell apoptosis continues to grow, proliferation and eventually leadto tumors. Bcl-xL and Survivin genes are apoptosis suppressor genes, the highexpression can restrain the cell apoptosis induced by many factors, it is alsofound that both participate in the formation of blood vessels in the process,which hint them with tumors, invaded and transfer closely related, therefore,bcl-xL and Survivin correlation has become a hot spot of research in recentyears.Colorectal cancer is the digestive tract of the common malignant tumor, inrecent years, its incidence significant rise in advance and the age have atendency, and has not been seen in colorectal cancer the combined testing.Thisstudy used immunohistochemical method, united detection analysis theexpression of Bcl-xL and Survivin in colorectal cancer organizations and therelationship between them and the colorectal cancer clinical pathologicalfeatures. Methods: All patients that were randomly elected in department ofcolorectal cancer surgery in Luzhou Medical College Hospital during2009.Oct.-2010, May.That were not treated by chemotherapy, radiotherapy, andbiotherapy before operation. Specimens of colorectal cancer in55cases, whichelected the complete collection of clinical and pathological data, detectedBcl-xL、 Survivin expression in cancer tissue and incisal edge by SPimmunohistochemistry. Investigating their expression in colorectal canceroccurrence and development in the role and analyzing the relationship betweenclinical pathological factors. For colorectal cancer invasion and metastasis ofprediction, prognosis of the judgment to provide the theory basis, meanwhile toreveal Bcl-xL and survivin mechanism, which laid the foundation of theinternal relations.Results:1The expression rates of Bcl-xL in colorectal cancer and peripheraltissues were70.90%（39/55）、10.90%（6/55）. The high expression were closelycorrelated with colorectal cancer differentiation degree, lymph node metastasis,TNM clinical stages (P <0.05).2The expression rates of Survivin in colorectal cancer and peripheraltissues were74.54%（41/55）、1.82%（1/55）.The high expression were closelyrelated to colorectal cancer differentiation degree, lymph node metastasis, TNMclinical stages (P <0.05).3Bcl-xL and Survivin in colorectal cancer tissue of expressing relationships, Bcl-xL and Survivin both the masculine expresser account for33cases in the55cases of colorectal cancer tissue, simultaneously the negativeexpresser ccounts for8cases,both expression were positively correlated（r=0.361,P=0.007）.Conclusion:1The overexpression of Bcl-xL was closely correlated with colorectalcancer differentiation degree, lymph node metastasis, TNM clinical stages,moreover have nothing to do with age, sex, tumor location, size, infiltrationdepth,and the expression rates of Bcl-xL were higher in the late clinical stage.This proved that Bcl-xL played an important role in the form and developmentof colorectal cancer.2The overexpression of Survivin was closely related to colorectal cancerdifferentiation degree, lymph node metastasis, TNM clinical stages, moreoverhave nothing to do with age, sex, tumor location, size, infiltration depth, and theexpression rates of Survivin were higher in the late clinical stage, which provedthat Survivin played an important role in the form and development ofcolorectal cancer.3The expression of Bcl-xl and Survivin were showed the highestconsistency in colorectal cancer tissue, and correlated with colorectal cancerdifferentiation degree, lymph node metastasis, and clinical stages. This provedthat Bcl-xL and Survivin common played antiapoptotic role in the form anddevelopment of colorectal cancer. 4It is suggested that Bcl-xl and Survivin united detection can be used as aprognostic colorectal cancer the judgment of the indexes and the effectivetargets of immune therapy.